- Semiautomated confocal imaging of fungal pathogenesis on plants: Microscopic analysis of macroscopic specimens
- Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
As reported in recent three publications, investigators at the University of Delaware use the ORIP-funded confocal microscope (S10OD016361) to study the morphology and activity of cellular organelles in the context of immunity and disease resistance. Another S10-awarded microscope (S10 RR027273) also served these experiments. In work funded by NIGMS grants (R01GM097587, P20GM103446), researchers investigated cellular mechanisms related to the release of immune signals, recognition of pathogens, and generation of defense responses. Using their own, novel, fluorescence-based methods for 3D cellular phenotyping and time-dependent host-pathogen-interactions combined with specialized computation tools for fluorescence imaging data analysis, researchers related organelle dynamics to molecular signaling.