Zebrafish Community Cre/lox Resource
Grant Numbers: R24OD020166 and R24OD036201
Research Emphasis/Objectives 
The mission of the Zebrafish Community Cre/lox Resource (CCR) is to provide the zebrafish community with Cre resources that open new areas of investigation and promote novel discoveries in human health, development, and disease. With CRISPR/Cas9 precision targeted integration, the Zebrafish CCR can generate Cre knock-ins and floxed alleles in any gene of interest, unlocking previously inaccessible cell lineages and cell type–specific gene studies to broaden the scope and impact of zebrafish research.
Current Research
The Zebrafish CCR provides precision Cre and tamoxifen regulated-CreERT2 knock-ins for spatial and temporal analysis of proneural, mesoderm, endoderm, vascular, blood, and hematopoietic stem cell lineages. The resource is building a set of UFlip Floxed conditional alleles in genes that regulate the epigenome and mitochondrial function, rapidly emerging areas crucial to development, disease, and regeneration. Floxed alleles allow simultaneous gene inactivation and mRFP labeling of mutant cells.
Services Provided
The Zebrafish CCR currently has 10 Cre/CreERT2 lines for proneural, mesoderm, endoderm, vascular, and hematopoietic stem cell lineages. Three floxed lines are available for cell cycle control and Hdac1-mediated epigenetic regulation of gene expression. The resource has seven floxed lines in progress for histone and DNA methylation regulation, and one floxed line for the nuclear encoded mitochondrial DNA replicase PolG. The resource is regularly updated as new lines become available. Requests for lines are made online through the Zebrafish CCR website.
Contact Information
3005 Advanced Teaching and Research Building
2213 Pammel Drive
Iowa State University
Ames, IA 50011-1101
515-294-4445
[email protected]
Principal Investigator
Maura McGrail
[email protected]
Additional Contact
Jeffrey J. Essner
[email protected]