Viper Resource Grant at Texas A&M University-Kingsville

Grant Number: P40 OD010960

Research Emphasis/Objectives

NNRTC Student at work in labThe National Natural Toxins Research Center (NNTRC), a component of Texas A&M University-Kingsville (TAMUK), is a unique animal and biological material resource center organized to support basic and translational research on venomous snakes and their venoms. Since the initiation of this P40 grant in 2003, the NNTRC has served as the only federally funded viper resource center in the United States, playing a critical role as a provider of high-quality single-source venoms and snake-related research materials to national and international biomedical and biological research programs. The goal of the NNTRC is to provide native venom and purified venom components, recombinant venom proteins, and specialized venom research services of the highest quality to support snake venom–related research in the United States and abroad.

To achieve its goal, the NNTRC will address the following three Specific Aims:

  • Aim #1: To operate the NNTRC as a resource center that provides high-quality venom and products that support biological and biomedical research for national and international research programs (70%).
  • Aim #2: To develop and expand the collection of snakes, specialized services, and outreach programs to support growth of venom-related research in the United States (20%).
  • Aim #3: To conduct a state-of-the-art applied research program to support the development of new venom-related research services.
    • Sub-Aim #1: To apply new methods and approaches to the production of recombinant toxins for rare and low-abundance components of crotalid venom.
    • Sub-Aim #2: To develop novel cell-based assays that can be used for the conventional and high-throughput testing of anti-venoms and toxin inhibitory molecules.

Services Provided

Products Provided

Services Provided

Venom (single or pooled source)

Venom lethality-LD50

Venom fractions

Antivenom efficacy-ED50

Snake sheds

Hemorrhagic assays

Snake blood

In vitro angiogenic assays

Snake glands

In vivo angiogenic assays

Other snake organs

In vivo antitumor assays

cDNA libraries

Cytotoxicity assays

Recombinant venom proteins/peptides

Cell proliferations assays

Snake venom extracellular vesicles and exosomes

Platelet function assays

Synthetic peptides

Enzyme-linked immunoabsorbant assay (ELISAs)

 

Western blots

 

1-D and 2-D SDS electrophoresis

 

Mass spectrometry

 

cDNA library construction

 

Cloning of venom molecules

 

Venom molecule purification

 

In vitro cell permeability assay

 

In vivo cell permeability assay (Miles assay)

 

Indirect hemolytic assay

 

In vitro blood coagulation assays

 

In vitro antibacterial activity (disc diffusion and microdilution methods)

 

Snake envenomation biomarker discovery

 

Snake venom extracellular vesicles and exosomes cytotoxicity analysis

 

Toxicity score analysis

 

Contact Information

National Natural Toxins Research Center
975 W. Avenue B, MSC 224
A.L. Kleberg Hall, Room 100
Texas A&M University-Kingsville
Kingsville, TX 78363
tamuk.edu/artsci/departments/nntrc/index.html

Co-Principal Investigator

Elda E. Sánchez, Ph.D.
Phone: 361-593-3796
elda.sanchez@tamuk.edu

Other/Resource Contacts

Peter J.A. Davies, M.D., Ph.D.
Phone: 713-677-7473
pdavies@tamu.edu

Other/Resource Contacts

Co-Investigator: Jacob A. Galan, Ph.D.
Phone: 361-593-3794
jacob.galan@tamuk.edu

Last updated: 08-18-2021