Zebrafish Embryo and Larvae Cryopreservation Resources
Grant Number: R24OD034189
Research Emphasis/Objectives
Zebrafish models are invaluable tools for understanding human health and disease and have taught us much of what we know about biology. Immense efforts have gone into the generation of transgenic, reporter, and mutant lines; however, maintaining live fish indefinitely has become a huge cost burden that also puts animals at risk. A laboratory at the Center for Engineering in Medicine & Surgery is using nature-inspired cryopreservation methods to increase the hypothermia tolerance of zebrafish embryos and larvae for long-term biobanking.
Services Provided
The laboratory is advancing cryopreservation techniques by systematically optimizing methods for introducing cryoprotective agents into zebrafish embryos and larvae. This approach merges established practices with innovative strategies to enhance preservation efficacy and cellular viability, ensuring the preservation of valuable transgenic lines and disease models for future research applications.
Contact Information
Center for Engineering in Medicine & Surgery
51 Blossom Street
Boston, MA 02114
Principal Investigator
Shannon N. Tessier, Ph.D.
Associate Professor, Harvard Medical School
Director of Surgical Research, Massachusetts General Hospital
Center for Engineering in Medicine & Surgery
Email: [email protected]
Website: https://www.massgeneral.org/surgery/cems/faculty-and-staff/shannon-tessier
Additional Contact(s)
Rasha Al-attar, Ph.D.
Research Fellow
Email: [email protected]