Vertebrate Models

Vertebrate models (e.g., rodents, swine, nonhuman primates) have long played a central role in biomedical research because they share much in common with humans with respect to genetics, development, physiology, behavior and disease. The DCM is interested in research that broadens the utility of human disease models.

Aquatics

Ambystoma Genetic Stock Center
P40 OD019794

Research Emphasis/Objectives

The Ambystoma Genetic Stock Center (AGSC) maintains a historically significant collection of Mexican axolotls (Ambystoma mexicanum), an endangered salamander that provides high quality stocks in support of biomedical research. Most notably, axolotls are studied because they are unique among vertebrates in being able to regenerate numerous tissues and body parts. The AGSC also serves as an informatics hub where investigators obtain information about axolotl biology, husbandry, and technical procedures. Currently, the AGSC is implementing a new genetic management plan, increasing production and distribution of axolotls, and optimizing methods to make transgenic axolotls.

Services Provided

Animals

The AGSC sells wild type axolotls as well as several color variants: white, albino, melanoid, and axanthic. Also, transgenic GFP and RFP expressing axolotls are available, as are developmental mutants (e.g. eyeless, cardiac, and short-toes). Axolotl embryos and early stage larvae are readily available; juveniles and adults are available in limited numbers. Investigators are encouraged to contact AGSC staff about available stocks and stocks that can be generated upon request.

Supplies

Axolotl husbandry supplies are available for purchase including food, salt and conditioners to make axolotl water, and bowls to rear axolotls. Also, axolotl supplies, kits, and aquaria are available to support K-12 educational activities and exhibit axolotls in public displays.

Microinjection

The AGSC is working to establish a microinjection service, where users will provide genetic constructs or mRNA for injection into single-cell axolotl embryos. Please contact AGSC staff about progress toward the implementation of this service.

Contact Information

Ambystoma Genetic Stock Center
University of Kentucky
Department of Biology
101 Thomas Hunt Morgan Building
Lexington, Kentucky 40506-0225

Principle Investigator

S. Randal Voss, Director
Phone: 859-257-9888
Fax: 859-2357-1717

Resource Contact

Laura Muzinic. Associate Director
Chris Muzinic, Curator
Phone: 859-323-5679
Fax: 859-257-1717
National Xenopus Resource Center
P40 OD010997

Research Emphasis/Objectives

The National Xenopus Resource (NXR) was established to maintain and generate critical animal stocks for the research community, disseminate new technology and optimize animal husbandry techniques for the research community. The NXR serves as a stock center for various transgenic, mutant and inbred Xenopus laevis and Xenopus tropicalis animals. The NXR also provides a custom transgenic and mutant resource to create new transgenic and mutant lines for individual labs. In addition to these services, in collaboration with the Marine Biological Laboratory (MBL), the NXR offers advanced training workshops and the ability for visiting scientists to perform short term research projects utilizing NXR resources.

Services Provided

Animals

Healthy stocks of inbred, mutant and transgenic Xenopus laevis and Xenopus tropicalis are maintained and distributed by the NXR. The majority of the lines currently housed by the NXR were donated by members of the Xenopus research community. The inbred Xenopus laevis J strain, whose genome has been sequenced, is also available.

Custom Research Services

The NXR will create custom transgenic and mutant X. laevis and X. tropicalis frogs for individual researchers as a low-cost service. Transgenic animals are made using the I-SceI meganuclease method, while mutant animals are created using CRISPR/Cas and TALEN methods. The NXR excels in breeding specialized frogs in a rapid and efficient manner allowing investigators to focus on other aspects of their research and leaving the husbandry to us.

Advanced Training Workshops

The NXR serves as a training venue for the community. In collaboration with the MBL the NXR hosts advanced training workshops and mini-courses tailored specifically to Xenopus techniques. These workshops are designed specifically with the Xenopus community in mind and are geared to complement and advance research in individual labs; topics covered include genome editing, advanced imaging, bioinformatics and husbandry. The NXR also anticipates teaching new methods as they are developed in Xenopus. In addition, the NXR hosts the biennial PI meeting that provides a valuable forum for discussion of current research, generating collaborations and for prioritizing community needs.

Research Facility Service

The research facility service offers researchers the opportunity to come to the NXR to perform short term research projects utilizing NXR resources. This is open to all scientists interested in performing experiments in Xenopus, regardless of whether they currently use frogs or not. Both Xenopus laevis and Xenopus tropicalis frogs are available. Lab space and housing are readily available at the MBL. The NXR also offers Xenopus housing to those investigators who come to the MBL in the summer and require Xenopus in their research.

Contact Information

National Xenopus Resource
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543

Principle Investigator

Robert Grainger, PhD
Phone: 434-982-5495

Director and Contact PI

Marko Horb, Ph.D.
Phone: 508-289-7627
Fax: 508-289-7900

Resource Contact

Marcin Wlizla, PhD
Phone: 508-289-7370
Research Resources for Model Amphibians
R24 OD010435

Research Emphasis/Objectives

Salamanders are important vertebrate model organisms in several areas of biomedical research, including tissue regeneration, neurobiology, and aging. The Salamander Genome Project (SGP) is developing genomic information and resources to better enable research efforts using the Mexican axolotl (Ambystoma mexicanum). Current research efforts include sequencing and assembling the large, axolotl genome and characterizing epigenetic modifications of chromatin and DNA during tissue regeneration.

Services Provided

Information about the axolotl genome and transcriptome is made available to the community via a website called Sal-Site. Investigators that have questions about axolotl biology or available resources are encouraged to contact the PI.

Contact Information

Salamander Genome Project
Department of Biology
University of Kentucky
Lexington, Kentucky 40506-0225

Principal Investigators

S. Randal Voss, Director
859-257-9888
Fax: 859-257-1717
 
Jeramiah J. Smith, Ph.D.
859-257-0124
Fax: 859-257-1717

 

Xiphophorus Genetic Stock Center
R24 OD011120

Research Emphasis/Objectives

The Xiphophorus Genetic Stock Center (XGSC) provides the research community with animals from 54 pedigreed parental lines, representing 24 species, in large numbers according to the specifications of each investigator. The Center has produced 29 distinct interspecies hybrid genetic crosses for genetic analyses. The Center serves as a catalyst for continued field studies aimed at identification and preservation of new Xiphophorus species before their demise due to habitat destruction. The XGSS serves as the bridge between production of genetically managed research animals and their eventual dissemination and use in research projects.

Current Research

The XGSC spearheaded efforts to produce the genome sequence and assembly for X. maculatus Jp 163 A (Schartl et al., 2013; Nature Genetics, 45: 567-572) and assisted the alignment of a Rad-Tag meiotic map (16,761 markers) with the genome assembly to produce one of the best gene maps for any vertebrate (Amores et al., 2014; Genetics, 197: 625-641). In addition, in collaboration with Drs. Wes Warren (Washington Univ. Genome Center,) John Postlethwait (Univ. Oregon) and Manfred Schartl (Univ. Wurzburg, GDR), the XGSC has also recently sequenced and assembled the genomes of X. hellerii, X. couchianus, and X. montezumae (Shen et al, 2016; BMC Genomics 17:376-50). The genomes sequenced represent parental fish that are used to produce at least six distinct interspecies hybrid experimental models for spontaneous and induced tumorigenesis. Ongoing efforts involve molecular genetic characterization of genes involved with UV induced melanoma development and genes that hallmark response to environmental stimulus such a varying wavelengths of light.

In collaboration with Dr. Terry Tiersch, Louisiana State University, the stock center has established technology to allow cryopreservation of Xiphophorus sperm samples and thereby provide a preservation program where the XGSC maintains the genetic heritage of all Xiphophorus strains and species (Yang et al., 2012; Zebrafish, 9:126-134).

Services Provided

Since its establishment more than 85 years ago, the XGSC has provided pedigreed fish from more than 50 genetic strains to scientists and aquarists around the world. These fish have been in continuous culture since their arrival at the XGSC, with some lines in their 114th generation of brother-sister pedigreed line breeding. Scientists in more than 40 laboratories in the United States, Singapore, Canada, Mexico, Japan, Colombia, and Germany work on Xiphophorus genetics and depend on strains available from the XGSC.

Backcross hybrid tissues and nucleic acid panels are being developed and will be available to investigators. Primer pair sequences corresponding to genetic markers and assay information for several interspecies crosses will be made available to the research community. Also, the XGSC can assist investigators with bioinformatics analyses of differential gene expression using Xiphophorus transcriptome and genomic resources.

In addition to supplying strains and providing consultation on husbandry and genetic questions, the XGSC produces custom interspecies hybrids (both first generation F1, and backcross hybrid generation BC1) for a variety of projects, shipping hundreds of such fish each year. The XGSC will assist researchers in performing detailed genetic analyses on fish samples where a collaborating researcher has scored a phenotype of interest.

Contact Information

Department of Chemistry and Biochemistry
Texas State University
419 Centennial Hall
San Marcos, TX 78666-4616

Principal Investigator

Ronald B. Walter, Ph.D.
Phone: 512-245-0357
Fax: 512-245-1922

Resource Contact

Markita Savage
Phone: 512-245-8469
Fax: 512-245-1922
Zebrafish International Resource Center
P40 OD011021

Research Emphasis/Objectives

The Zebrafish International Resource Center (ZIRC) is a central repository for materials and information about zebrafish research, as well as a stock center for wild-type and mutant strains of zebrafish (Danio rerio). Materials and zebrafish strains are distributed to the research community. Pathology services are provided for diseased fish. Standards and procedures for maintaining healthy strains of zebrafish are being developed, and a manual for prevention, diagnosis, and treatment of diseases affecting zebrafish is available.

Services Provided

Animals

Healthy stocks of zebrafish and frozen sperm are maintained and distributed to the research community. Strains of wild-type fish and lines carrying mutations and transgenes are accepted from the research community, maintained, and distributed on request.

Biological Materials

Antibodies, gene probes, and other markers to analyze wild-type and mutant stocks are received, stored, and distributed.

Genotyping

Genotyping protocols are provided for mutant and transgenic lines.

Pathology

Advice about zebrafish health is provided. Diseased fish and tissue samples may be sent to the resource center for pathology analysis. The center also provides routine sentinel or quality control testing of zebrafish from healthy laboratory colonies (e.g., to fulfill IACUC health status monitoring).

Training

Researchers are welcome to visit the resource center to learn about the aquaculture systems and husbandry and health procedures.

Contact Information

Zebrafish International Resource Center
5274 University of Oregon
Eugene, OR 97403-5274

Principal Investigator

Monte Westerfield, Ph.D.
Phone: 541-346-4607
Fax: 541-346-4548
monte@uoneuro.uoregon.edu (link sends e-mail)

Resource Contact

Erin Quinn
Phone: 541-346-6028
Fax: 541-346-6151
erin@zfin.org (link sends e-mail)

Nonhuman Primates

(National Primate Research Centers)

Overview

The goals of the National Primate Research Center (NPRC) program is to facilitate the effective use of nonhuman primates (NHPs) by scientists engaged in biomedical research. The NPRC program complements and enables the missions of the other NIH Institutes and Centers (ICs) by providing the animals, facilities, expertise, and resources required to enable NHP research in specific disease areas. ORIP’s Division of Comparative Medicine (DCM) funds seven NPRCs, which are centralized facilities in various parts of the country, and are available to investigators on a national basis. Individual NPRCs have specific areas of emphasis, but each is expected to provide a variety of services both individually and through inter-NPRC collaborations to a wide range of investigators. The majority of researchers, who use the NPRC physical and intellectual infrastructure, are funded by the U.S. Public Health Services, particularly NIH ICs. The NPRCs also support research funded by other federal agencies, non-profit foundations, and the private sector. Each NPRC has a Pilot Research Program, which is advertised nationally and available to investigators who propose to the use of NHPs in developmental projects in biomedical research or for studies enhancing the welfare or husbandry of NHPs. For additional information on the seven NPRCS, see the links below or visit the NPRC Research and Capabilities website. Additional information is available on NPRC Access Criteria and Procedures.

NPRC Access Criteria and Procedures

The National Primate Research Center (NPRC) facilities and resources enable NPRC staff scientists and investigators from the host institution and others across the country to collaborate on their research projects. The centers' specialized resources are intended to support investigators who receive their primary research project funding from NIH, but they also may be used by investigators who are funded by other federal, state, and local agencies, as well as by research foundations and the private sector.

Each NPRC has a Visiting Scientist Program that offers advanced training and research in nonhuman primate biology. Collaborative arrangements between investigators and center scientific staff are encouraged and can be developed on studies related to major human diseases, subject to the availability of resources. Nonhuman primate blood samples, organs, and biological fluids are available through the NPRCs. The following standardized criteria and procedures have been implemented at each NPRC to facilitate utilization of center resources:

Access Criteria

  • The nature and scope of the proposed research must be best conducted with nonhuman primates and be compatible with available center resources.
  • The proposed research must have high scientific merit as determined by peer review.
  • NIH–funded research takes precedence over research activities funded by other sources.
  • Grants must contain appropriate budgets for the NPRC portion, including animal per diem costs.
  • Availability of NPRC resources, including animals, space, research services and support, and special requirements—such as biosafety facilities—are also limiting factors that must be considered by the investigator.
  • Because of potential contamination (e.g., viral, microbial), movement of animals into or out of the NPRC facilities is not allowed. Thus, the proposed research using live animals must use NPRC animals, and the research must be conducted at the NPRC.

Access Procedures

  • An initial research proposal must be submitted by the researcher to the NPRC prior to submitting an application for funding. The director then consults with the research services, veterinary, and colony management staff members at the center to assess resource availability and project feasibility.
  • When resource availability and project feasibility have been established, the NPRC staff will provide budget information to the researcher regarding the center costs to be included in the formal research proposal.
  • The scientific merit of the proposal must then be evaluated through the NIH peer review process or through a similar process at other agencies. However, small pilot projects with other funding sources may be considered. In the latter case, the peer review is conducted by the NPRC Research Advisory Committee.
  • In addition to the scientific peer review, a protocol approved by the Institutional Animal Care and Use Committees (IACUC) at both the investigator's institution and the NPRC must be in place. Protocols must also be established to address biosafety concerns.
  • When the investigator has received notification of funding, the NPRC director should be advised immediately so that the resources at the center may be reserved for the funded proposal.
  • Biological materials such as blood samples, organ tissues, and biological fluids can be obtained by contacting the directors and staff of the NPRCs.

All publications resulting from research conducted at or with NPRC resources must bear an appropriate acknowledgment of ORIP support.

Inquiries

For additional information about the Visiting Scientist Program and resources available at a specific center, including applying to utilize a center's resources, contact the center director or appropriate contact person listed in this directory.

California National Primate Research Center
P51 OD011107

Research Emphasis/Objectives

The California National Primate Research Center (NPRC) is a research unit of the University of California, Davis. The mission of the CNPRC is to improve human health and quality of life through support of exceptional nonhuman primate research programs.

Current Research

The center has a diverse program of research utilizing nonhuman primates. Research projects encompass many aspects of biology and medicine, including AIDS and other infectious diseases; reproductive issues such as those associated with conception, pregnancy, and fetal growth and development; neurodegenerative conditions such as Alzheimer's disease; nutritional deficiencies; pulmonary disorders such as asthma, emphysema, and other chronic obstructive lung diseases; xenotransplantation; cell- and gene-based therapies; acute and chronic stress; temperament and biobehavioral organization; social relationships; neurobiology; cognitive function; and behavioral development.

Services Provided

Research units include brain, mind, and behavior; reproductive sciences and regenerative medicine; respiratory diseases; and virology and immunology; as well as an affiliate research program, core services, and the primate services and medicine division. Research opportunities are available for investigators from national and international institutions, as well as scientists within the UC Davis research community. The NPRC is also home to the Center for Fetal Monkey Gene Transfer for Heart, Lung, and Blood Diseases under the direction of Alice Tarantal, Ph.D.

To Outside Investigators

Specimens

Organs and tissues are provided when available; other biological samples are provided on special request. Shipping, collecting, and processing costs are charged to the requestor.

To Collaborating Scientists

  • Scientists wishing to conduct research at the center must have their projects reviewed and approved by the center director, research advisory committee, and campus animal care review committee. The center's services are available to collaborating scientists on a fee-for-service basis. Services include:

  • Core Services

    Behavior Management Services Core
    Endocrine Core
    Immunology and Pathogen Detection Resources Core
    Inhalation Exposure Core
    Multimodal Imaging Core

    Contact Core Leaders at http://www.cnprc.ucdavis.edu/our-services/core-services/

  • Primate Medicine

    Preventive medicine and epidemiologic evaluation, surgery, radiology, therapeutics, specialized medical procedures.

  • Diagnostic Pathology and Clinical Laboratory Services

    Bacteriology, biochemistry, hematology, parasitology, pathology, virology.

  • Animals

    Center breeding colony: rhesus macaque (M.mulatta). Center research colony: Callicebus cupreus, M. mulatta.

Contact Information

California National Primate Research Center
University of California, Davis
Davis, CA 95616

Principal Investigator

Cameron S Carter. M.D.
UC Davis Interim Vice Chancellor for Research

Center Director

John Morrison, Ph.D.
Phone: 530-754-4829

Additional Contact

Jeffrey A. Roberts, DVM
Associate Director, Primate Services
Phone: 530-752-6490
Oregon National Primate Research Center
P51 OD011092

Research Emphasis/Objectives

The Oregon National Primate Research Center (ONPRC) is engaged in a spectrum of studies based in the scientific research divisions of Diabetes, Obesity & Metabolism, Neuroscience, Pathobiology & Immunology, and Reproductive & Developmental Sciences. This research is complimented by interdisciplinary programs in the areas of addiction, childhood health, healthy aging and primate genetics. Collaborative research that is initiated by external investigators is managed through the Collaborative Research Unit (CRU).

Current Research

  • Division of Cardiometabolic Health: Diet-induced maternal obesity, metabolic syndrome and insulin resistance, adipose biology, and islet function.
  • Neuroscience: Research on fundamental and integrative mechanisms underlying nervous system dysfunctions and resultant disease states using the major scientific disciplines of neuroendocrinology, neurodevelopment, neurodegeneration, addiction, aging, and primate genetics. Specific technologies are produced and utilized, including novel methods to acquire in vivo imaging data, measure cognitive performance, introduce and assess genetic therapeutics, provide functional neuroanatomical links to behavior, and identify informative phenotypes for genetic analysis of traits.
  • Pathobiology & Immunology: Cellular and molecular events controlling pathogenesis and immune responses of clinically important infectious agents (HIV/SIV, herpes family viruses, Mycobacterium tuberculosis, yellow fever virus, Dengue, Chikungunya virus), novel vaccine development, and research in basic primate immunology, immune senescence and bioterrorism.
  • Reproductive & Developmental Sciences: Regulation of neuroendocrine, gonadal, reproductive tract and gamete function as related to advancing our understanding of reproductive physiology, the diagnosis and treatment of infertility, the creation of novel contraceptives, as well as defining the genetic and epigenetic parameters necessary for normal growth and development.

Other key scientific programs

  • Healthy Aging: Dietary modulation of age-related changes in immune function, circadian physiology, and learning and memory, efficacy of endocrine therapies for postmenopausal declines in cognition and emotional health.
  • Early Childhood Health and Development
  • Primate Genetics

Animal Colony

Rhesus macaques (Macaca mulatta), ~4,200; Japanese macaques (Macaca fuscata), ~350; cynomolgus macaques, ~100; Baboons (Papio anubis), relatively few. Specialized animal resources: Obese resource (Japanese and rhesus macaque models of diet-induced obesity); Aging resource (aged rhesus macaque cohort); Primate Genetics Program (colony ancestry, pedigree genetics, demographics and biostatistics); Behavioral Sciences Unit; Timed Mated Breeding Program; Infectious Disease Resource; Japanese macaque resource; Infant Lab; and an ABSL-3 animal research facility. Colony care and maintenance are the responsibility of the Division of Comparative Medicine, which includes 15 full-time veterinarians and ~110 support staff.

Services Provided

To Outside Investigators

Tissue specimens, organs, etc., as available from Pathology Services tissue procurement program. Costs are assumed by the requestor.

To Collaborating Scientists

Scientists wishing to conduct research at the ONPRC must have their projects approved by the Institutional Animal Care and Use Committee, the Institutional Biosafety Committee, and the Research Advisory Committee (RAC). The RAC does not typically evaluate proposals that undergo formal peer review by national review panels (i.e., NIH, DOD, etc.). Investigators interested in collaborative work who do not have an existing relationship with an ONPRC investigator should direct enquiries to the CRU, which is headed by the Associate Director for Research (contact information below). Collaborators have access to ONPRC research support cores, which are listed below. Most services are provided on a fee-for-service basis.

Pathology Services tissue procurement program

Necropsies, tissue distribution, consultation.

Imaging and Morphology

Confocal and laser-capture microscopy and stereology, tissue embedding and sectioning, immunohistochemistry, in situ hybridization.

Endocrine Technology Services

Steroid and protein hormone Luminex, ELISAs, IRMAs, and RIAs, assay development.

Molecular and Cellular Biology

DNA synthesis and sequencing, cDNA probes, real-time PCR, maintenance of cell lines, media preparation, lentivirus design and preparation.

Assisted Reproductive Technologies

Procedures related to in vitro fertilization, nuclear transfer, intracytoplasmic sperm injection, culture media, gamete preservation.

Magnetic Resonance Imaging

3T Siemens magnet, magnetic resonance spectroscopy, MRI training.

Flow Cytometry

Flow cell analysis and sorting.

Molecular Virology

Virus identification, production, and quantification, reagent and standardized assay development.

Primate Medicine

Preventive medicine and epidemiologic evaluation, surgery, radiology, therapeutics, specialized medical procedures.

Diagnostic Pathology and Clinical Laboratory Services

Bacteriology, biochemistry, hematology, parasitology,pathology, virology.

Animals

Center breeding colony: rhesus macaque ( M.mulatta). Center research colony: Callicebus cupreus, M. mulatta.

Contact Information

Oregon National Primate Research Center
Oregon Health & Science University
505 N.W. 185th Avenue
Beaverton, OR 97006

Principal Investigator

Joseph Robertson, M.D.
President, Oregon Health & Science University
Portland, OR 97239

Resource/ Additional Contacts

Center Director and Contact
Nancy L. Haigwood, Ph.D.
Phone: 503-690-5500
Fax: 503-690-5569

Associate Director for Research

Charles Roberts, Ph.D.
Phone: 503-690-5259
Fax: 503-690-5569
Southwest National Primate Research Center
P51 OD011133

Research Emphasis/Objectives

The Southwest National Primate Research Center (SNPRC) supports studies of nonhuman primate models of human diseases, including common chronic diseases and infectious diseases, and the effects that genetics and the environment have on physiological processes and susceptibility to specific diseases.

Current Research

Chronic Diseases

Genetic and environmental bases for susceptibility to atherosclerosis, hypertension, osteoporosis, obesity, and cancer; construction of baboon and rhesus gene maps; genome scans for disease-related genes; therapeutic monoclonal antibodies; development of new genetic analysis strategies and software.

Infectious Diseases and Biodefense

AIDS; hepatitis B, C, and E; herpes B and other herpes viruses; Chagas disease; hemorrhagic fevers; emerging viral diseases; vaccine and drug development and testing.

Development and Aging

Contraception; nutrient restriction or obesity in pregnancy and developmental programming; ingestive behavior; brain imaging; gene and stem cell therapies.

Services Provided

To Outside Investigators

The SNPRC encourages the use of its resources by investigators from the national and international biomedical research communities. The SNPRC is also available for collaborative research initiatives involving center staff and outside investigators. In general, expenses are assumed by the initiating investigator, and collaborative research efforts are covered by grants acquired collaboratively.

Specimens

Banked serum, tissue, and DNA samples; fresh blood, serum, plasma, tissues, and organs.

Animals

Baboons, (Papio hamadryasanubis, P.h. cynocephalus), chimpanzees (Pan troglodytes), rhesus macaques (Macaca mulatta), common marmosets (Callithrix jacchus), miscellaneous primate species as required for specific research purposes. Also, rhesus macaques with an LDL receptor defect that causes familial hypercholesterolemia.

Veterinary Technical Services

Timed pregnancies, tether, radiography, sonography, endoscopy, experimental surgery, experimental diets, nursery, behavioral assessment. ABSL-3 and ABSL-4 laboratories are available for infectious disease research, including research with Select Agents.

Pathology Services

Necropsies, clinical chemistry, hematology, histology, bacteriology, virology, parasitology.

Immunology Services

Flow cytometry, cytokine and hormone Luminex assays, ELISA, ELISPOT, viral screening, cell separation.

Data Services

Colony database system, genetic analysis software, genetic typing services.

Contact Information

Texas Biomedical Research Institute
Southwest National Primate Research Center
P. O. Box 760549
San Antonio, TX 78245-0549

Principal Investigator

Robert W. Gracy, Ph.D.
President and Chief Executive Officer

Resource/ Additional Contacts

Center Director and Contact
Robert E. Lanford, Ph.D.
Phone: 210-258-94450
Tulane National Primate Research Center
P51 OD011104

Research Emphasis/Objectives

The Tulane National Primate Research Center is heavily focused on infectious disease research and also has a significant program in gene therapy/regenerative medicine that capitalizes on a unique colony of macaques with Krabbe disease.

Current Research

The major areas of infectious disease research at the Center are currently AIDS, Lyme disease, Tuberculosis and biodefense-related agents. The AIDS-related research is quite diverse, covering pathogenesis, vaccine development, microbicides, and the origins of AIDS. These are multidisciplinary studies involving investigators in several Divisions at the TNPRC and collaborators outside the Center. Common to these studies is a focus on disease pathogenesis and on using such findings to inform the development of vaccines, diagnostics and therapeutics. The regenerative medicine program involves both traditional viral-vector-mediated gene transfer as well as nonhuman primate mesenchymal and embryonic stem cells.

Services Provided

To Outside Investigators

Specimens: Tissue specimens, blood, and other bodily fluids are provided when available. Collection, processing, and shipping costs are normally assumed by the requestor.

To Collaborating Scientists

The TNPRC provides highly integrated clinical and laboratory support for studies using nonhuman primates. This includes a full-time staff of clinical veterinarians and technicians and core services commonly used for infectious disease and gene therapy research including: 1) Diagnostic Parasitology; 2) Vector-Borne Diseases (maintains arthropods that are important for the study of vector-borne diseases); 3) DNA Microarray and Gene Expression; 4) Anatomic Pathology; 5) Clinical Pathology; 6) Molecular Pathology; 7) Confocal Microscopy and Image Analysis; 8) Flow Cytometry; 9) Cellular Immunology; 10) Virus Characterization, Isolation, and Production; 11) Pathogen Detection and Quantification; 12) Infectious Disease Aerobiology; 13) Genetics and Genome Banking; and 14) Vector Development and Production. Vector Development and Production; and 15) Nonhuman Primate Stem Cell Production.

Animals

Rhesus macaques (Macaca mulatta) of both Indian and Chinese origin are available. Other species can be obtained. The vast majority of the rhesus macaques are specific-pathogen free (B virus, SIV, SRV, and STLV1 negative).

Contact Information

Tulane National Primate Research Center
18703 Three Rivers Road
Covington, LA 70433

Principal Investigator

L. Lee Hamm, M.D.
Senior Vice President and Dean
School of Medicine

Resource/Additional Contacts

Center Director and Contact
Andrew A. Lackner, DVM, Ph.D., Dipl. A.C.V.P.
Phone: 985-871-6201
Fax: 985-871-6569

Additional Contact

Rudolf P. Bohm, Jr., DVM, Dipl. A.C.V.P.
Phone: 985-871-6362
Washington National Primate Research Center
P51 OD010425

Research Emphasis/Objectives

The Center’s mission is to support outstanding biomedical research directed towards significant human health issues and nonhuman primate health and biology. The Center’s scientists conduct a wide variety of fundamental and translational research protocols, investigate NHP biology and disease, support a broad affiliate scientist program, develop innovative technologies, and provide training to the scientific community and public.

Current Research

AIDS-Related Diseases

Providing expertise and resources to better understand, prevent and treat HIV and AIDS. Scientists provide contributions to AIDS research including characterization of the virus-host interaction, restriction factors, immune response, and development of new vaccines and therapeutics.

Nonhuman Primate Systems Biology

Using high-throughput molecular profiling, statistical analysis and computational modeling to understand infection, pathogenesis, and immunology.

Global Programs

Focusing on conservation biology, field study training and international outreach activities. Areas of focus include NHP population status, genetic characterization, habitat viability, population management, disease risk and sustainability modeling.

Neuroscience

Using the NHP model to answer questions about the nervous system, vision and more. Current research includes applications of gene therapy toward color vision research, exploration of treatments for spinal cord injuries and paralysis using electronic neurochips, and treatments for balance disorders using implanted devices.

Reproductive & Developmental Sciences

Exploring reproductive biology and cognitive development in NHPs.

Services Provided

Affiliate Scientists/Outside Investigators

The Center is committed to providing complete access to resources for the research community to facilitate all aspects of nonhuman primate-related research. The Center provides substantial assistance for collaborative research projects, including scientific and technical assistance with protocol development, grant submission, data collection and interpretation, and manuscript preparation.

Primate Resource Referral Service (PRRS)

The Primate Resource Referral Service (PRRS) provides the communications/database network needed for efficient acquisition and sharing of existing captive NHPs and primate-related resources by investigators and institutions both nationally and internationally. The overall goal of this service is to maximize the use of existing captive primates, thereby reducing the total number of NHPs needed for research, and in turn, helping to promote the conservation of primate populations. See Primate Resource Referral Service.

Pathology and Tissue Distribution Program

The Pathology and Tissue Distribution Program provides NHP tissue samples to a broad variety of biomedical scientists. This program is an NPRC leader in distribution of biologic samples to both academic and commercial biomedical researchers. Staff continue to modify tissue extraction, preservation, and shipping techniques to meet research needs and extend the use of this valuable NHP resource.

Infant Primate Research Laboratory (IPRL)

The IPRL provides services to investigators using infant NHPs as animal models for behavioral and biological research. The IPRL is supported as a core facility of both the WaNPRC and the UW Center on Human Development and Disability. The IPRL provides around-the-clock care for pregnant females and infants in order to optimize survival and minimize morbidity. This effort increases the number of healthy animals available to the overall primate colony and reduces costs to investigators. In addition, the Center has the ability to produce gestation-known fetuses and infants through the Timed Mating Breeding Program.

Animals

Pigtailed macaque (Macaca nemestrina), Cynomolgus macaque (Macaca fascicularis), rhesus monkey (Macaca mulatta), squirrel monkey (Simia sciureus)

Contact Information

Washington National Primate Research Center
University of Washington
I-421 Health Sciences
Box 357330
Seattle, WA 98195-7330

Principal Investigator

David M. Anderson, D.V.M
Executive Director
Health Sciences Administration

Resource/ Additional Contacts

Center Director and Contact
Michael J. Mustari, Ph.D.
Phone: 206-543-0440
Fax: 206-616-6771

Tissue Distribution

Wisconsin National Primate Research Center
P51 OD011106

Research Emphasis/Objectives

The WNPRC's mission is to increase our understanding of basic primate biology and to improve human health and quality of life through research. To accomplish this, the WNPRC:

  • Helps discover treatments, preventions and cures for human disease.
  • Generates new knowledge of primate biology, from the molecular and whole animal levels to the understanding of primate ecosystems.
  • Facilitates research progress by providing expertise, resources and training to scientists worldwide.
  • Collects primate information and disseminates to the research community and to the public.

Quick Facts:

  • Personnel: The WNPRC has a staff of 200 that supports research by more than 520 scientists and their personnel from the University of Wisconsin-Madison, the United States and around the world. More than 80 UW-Madison graduate students and post-doctoral trainees conduct research through the center each year.
  • Funding: Scientists working on more than 100 subprojects through the center annually earn awards totaling approximately $26 million – 88% in federal funding and 12% in non-federal sources.
  • Colony: 1,400 animals: 1,000 rhesus macaques, 200 common marmosets, 200 cynomolgus macaques.

Current Research

The WNPRC focuses on four strategic areas of research and a diverse affiliate program.

Global Infectious Disease (GID): Transmission and pathogenesis of Simian Immunodeficiency Virus (SIV), influenza, Dengue, viral escape, vaccine development, MHC-defined animals, influenza, and identification of new viruses with zoonotic and/or pandemic potential.

Regenerative and Reproductive Medicine (RRM): Embryonic/pluripotent stem cell biology including cellular therapies for hematologic, cardiovascular, and neurodegenerative diseases, organ transplant tolerance, stem cell-based therapies for AIDS; assisted reproductive technologies (ART) for NHP transgenesis, maternal-fetal health including pregnancy loss and poor outcomes, intrauterine environment in metabolic and reproductive epigenetic programming, endometriosis, and polycystic ovary disease.

Energy Metabolism and Chronic Disease (EMCD): Chronic disease and aging research, with an emphasis on the genetic, cellular, and whole animal effects of caloric restriction (CR), as well as excess caloric intake resulting in obesity and metabolic syndrome; diabetes mellitus, osteoporosis, and new studies on post menopausal hormone changes and metabolic disease risks.

Neuroscience: Preclinical Parkinson’s disease research, translational studies of glaucoma, as well as stress, anxiety, and depression, and basic studies of central nervous system mechanisms controlling fertility, puberty, menopause, and body weight, and neuroendocrine regulation of reproductive and social behaviors.

Services Provided

To Affiliate Scientists/Outside Investigators

Researchers interested in conducting research through the WNPRC and using any of the below services or resources should first contact the Scientific Protocol Implementation (SPI) Service, which integrates the expertise of Research Services and Animal Services to help researchers conduct leading edge science supported by expert animal care.

SPI integrates the expertise of Research Services and Animal Services to help researchers conduct leading edge science supported by expert animal care.

Research Services and Resources:

  • Assay Services
  • Elite Controller Resource
  • Genetics
  • Immunology
  • Virology
  • Aging Resource
  • Stem Cell Resource

Animal Services

  • Scientific Protocol Implementation
  • Behavioral Management Unit
  • Colony Management Unit
  • Compliance and Training Unit
  • Pathology Services (Tissue Distribution Program)
  • Veterinary Services Unit

To Collaborating Scientists

The center actively encourages national and international researchers to use its facilities and services and to conduct collaborative studies. Scientists wishing to conduct research must have their projects reviewed and approved by the center director and advisory committees and have independent funding to cover costs. Most of the center's services are available on a fee-for-service basis.

Contact Information

Wisconsin National Primate Research Center
1220 Capitol Court
Madison, WI 53715-1237

Principal Investigator

Marsha R. Mailick, Ph.D.
Vice Chancellor for Research
And Graduate Education

Resource/Additional Contacts

Center Director and Contact
Jon Levine, Ph.D.
Phone: 608-263-3500
Fax: 608-265-2067

Additional Contacts

Jordana Lenon
Public Information and Outreach
Phone: 608-263-7024
Yerkes National Primate Research Center
P51 OD011132

Research Emphasis/Objectives

The Yerkes National Primate Research Center conducts biomedical and biobehavioral research to improve the health and well-being of human and nonhuman primates.

Current Research

Microbiology and Immunology

Advance molecular and biological processes for understanding, preventing, and treating infectious diseases; primate models for research on AIDS pathogenesis, treatment, and vaccines; and for other infectious diseases including malaria and tuberculosis (TB).

Behavioral Neuroscience and Psychiatric Disorders

Conducts basic and translational research to understand the neurobiological mechanisms underlying behaviors relevant to developmental and psychiatric conditions such as autism spectrum disorders, anxiety-related disorders, depression, and post-traumatic stress disorders (PTSD).

Developmental and Cognitive Neuroscience

Examines the neurobiology of social behavior and cognition across the life span; genetic, biological, and environmental factors that regulate social behavior and cognition; how social experience affects physiological processes and brain function.

Neuropharmacology and Neurological Diseases

Focuses on neurochemistry and neuroanatomy of neurologic disease; mechanisms of drug addiction and dynamics of drug receptors; cognitive changes with aging and neurologic disease; oculomotor processing under normal and pathological conditions.

Services Provided

To Outside Investigators

Research proposals by investigators from other institutions are encouraged. Proposals should be submitted for review by the Scientific Advisory Committee (SAC) to ensure that resources are available. All proposals are reviewed by the Institutional Animal Care and Use Committee. Services available to outside investigators at approved rates include veterinary medicine, pathology, and biomedical engineering.

Animals

Rhesus macaque (Macaca mulatta), pigtailed macaques (M. nemestrina), cynomolgus macaque (M. fascicularis), sooty mangabey (Cercocebus atys), squirrel monkey (Saimiri sciureus), chimpanzees (Pan troglodytes).

Service Cores

Brain Imaging and Radiochemistry, Molecular Pathology, Center for AIDS Research (CFAR) Virology, Genomics, Biomarkers, NIH Tetramer, Confocal Microscope, Comparative AIDS Core, Flow Sorting, Rodent Vivarium.

Contact Information

Yerkes National Primate Research Center
Emory University
954 North Gatewood Road, N.E.
Atlanta, GA 30322

Principal Investigator

S. Wright Caughman, M.D.
Executive Vice President for Health

AffairsResource/Additional Contact

Center Director and Contact
R. Paul Johnson, M.D.
Phone: 404-727-7707
Fax: 404-727-0623

(Primate Research Resources)

Baboon Research Resources
P40 OD010988

Research Emphasis/Objectives

The Baboon Research Resource conducts multidisciplinary studies on captive baboons and provides a resource of laboratory-born and laboratory-reared baboons for NIH-sponsored research programs. Additional objectives are to: maintain and provide available research facilities accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International; serve as a ready source of baboons (Papio spp.) of mixed ages and sex for use in biomedical and behavioral sciences; and provide professional staff necessary to support investigators' research needs.

Current Research

Current research activities involve characterizing the endogenous microorganisms of the conventional research baboon, improving methods for production of baboons in a captive environment, developing a specific-pathogen-free colony of baboons, developing vaccines, and testing genetic diversity among the baboon breeding population.

Services Provided

To Outside Investigators

The mission of the Oklahoma University Health Sciences Center (OUHSC) Baboon Research Resource is to support biomedical and behavioral research requiring the baboon as the animal model. The resource supports research investigators at the OUHSC and also serves as a national resource by supporting numerous investigators located at institutions across the United States. This resource enables NIH-funded investigators to purchase baboons for their research programs, subcontract with the resource to conduct the study on location at the OUHSC, or lease the baboons for conducting their studies and then return the baboons to the breeding colony. Together with the Oklahoma State University College of Veterinary Medicine, the Oklahoma State University Center for Health Sciences, and the Texas Tech University Health Sciences Center, the resource continues to further develop and improve the usefulness of the baboon as an animal model.

To Collaborating Scientists

Individuals interested in collaborative studies must provide a protocol to the principal investigator of the Baboon Research Resource. Approval of collaborative projects depends on the relevance of the proposed project to the objectives of the Baboon Research Resource, with preference given to NIH-funded studies. Complete animal husbandry, veterinary medical care, technical assistance, and pathology services are available to investigators who have approval from the principal investigator to use resource colony animals.

Animals

Adult, infant, and juvenile baboons are available.

Contact Information

Baboon Research Resources
University of Oklahoma Health Sciences Center
Division of Animal Resources
940 S. L. Young Boulevard, BMSB 203
Oklahoma City, OK 73190

Principal Investigator

Gary L. White, DVM, M.M.S.
Phone: 405-271-5185
Fax: 405-271-2660

Resource/Additional Contact

Richard W. Eberle, Ph.D.
Phone: 405-744-816
Fax: 405-744-5275
Caribbean Primate Research Center Program
P40 OD012217

Research Emphasis/Objectives

The Caribbean Primate Research Center Program (CPRC) is committed to provide to the Scientific Community, Indian-origin rhesus macaque with known background and of the same genetic pool, for the studies of numerous diseases that afflict humans, and to conduct multidisciplinary collaborative studies on the life cycle of the rhesus macaques as a biological model for humans.

CPRC Divisions

Cayo Santiago

The Cayo Santiago (CS) population is the oldest free-ranging colony that is maintained under minimal human intervention and since its establishment in 1938 when 409 founders from India were released onto Cayo Santiago, the island population remains a closed colony. Replacements since the introduction of the original stock have been exclusively recruited through births. There is an extensive computerized demographic database which contains up to 11 macaque generations traced through matrilines. A genetics database contains genotypes and paternity for all monkeys born since 1990, as well as many of the older animals. It serves a platform for short- and long-term studies of social and sexual behavior, population genetics, demography, reproductive biology, psychopharmacology, functional morphological, and parasites of rhesus monkeys maintained under semi-natural conditions.

Sabana Seca Field Station

Location of the headquarters of the CPRC, specific-pathogen-free (SPF) rhesus breeding colony, and biomedical research on spontaneous diseases (arthritis, osteoporosis, adult-onset macular degeneration, glaucoma, diabetes, obesity, hypertension), social behavior, endocrinology, medical genetics, vaccine development, and husbandry of Cayo Santiago-derived rhesus macaques.

Laboratory for Primate Morphology (LPM)

Headquarters of the CPRC, specific-pathogen-free (SPF) rhesus breeding colony and biomedical research on spontaneous diseases (arthritis, osteoporosis, adult-onset macular degeneration, glaucoma, diabetes, obesity, hypertension), social behavior, endocrinology, medical genetics, vaccine development, and husbandry of Cayo Santiago-derived rhesus macaques.

Virology Laboratories

Research on dengue and Zika using a NHP model developed in our Institution. We also support multiples vaccine projects on dengue and simian immunodeficiency virus (SIV, as a model for AIDS in rhesus monkeys). This BL2/3 fully equipped laboratory also supports the SPF grants by performing serological testing of herpes, STLV-1, SRV, and SIV in rhesus macaques.

Translational Science Initiative at CPRC

The research activities are conducted throughout the Translational Science Initiative (TSI). The mission of TSI is to catalyze the generation of innovative models and technologies that will enhance the development, testing and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions. A major strength of this TSI is conducting multidisciplinary collaborative studies to establish and to validate NHP models for preclinical translational sciences covering different humans’ diseases. The TSI emphasizes research on Dengue virus and the SIV (as a model for AIDS), its pathogenesis, strategies for vaccine development, and immune-based therapeutic strategies. Models for stroke, septic shock, and diabetes in rhesus are also investigated among others.

One Health Approach

The CPRC supports and promotes the One Health Initiative as a movement to promote all-inclusive collaborations between physicians, veterinarians, dentists, nurses and other scientific-health and environmentally related disciplines. Most of translational research project conducted at the CPRC are results of collaborations among researchers with different professional backgrounds. The initiative is also advocated in the protection of the environment. For its location, the CPRC is surrounded by wetland. This area of study is the natural habitat of the critically endangered Puerto Rican plains coqui frog, Eleutherodactylus juanariveroi.

Services Provided

CPRC welcomes collaborative research with established behavioral and biomedical investigators and encourages the use of its animal and osteological resources for dissertation research. All proposals receive rigorous peer review and are judged on scientific merit, feasibility, and potential overlap with ongoing studies. Protocols using live monkeys must be approved by the Institutional Animal Care and Use Committee (IACUC) of the home institution, as well as the University of Puerto Rico Medical Sciences Campus IACUC.

Collaborations

CPRC welcomes collaborative research with established behavioral and biomedical investigators and encourages the use of its animal and osteological resources for dissertation research. All proposals receive rigorous peer review and are judged on scientific merit, feasibility, and potential overlap with ongoing studies. Protocols using live monkeys must be approved by the Institutional Animal Care and Use Committee (IACUC) of the home institution, as well as the University of Puerto Rico Medical Sciences Campus IACUC.

Specimens

Tissue specimens, organs, blood, skeletal structures, etc.

Animals

SPF and conventional rhesus monkeys are available to NIH Research Investigators. We are the second largest provider of conventional and SPF Indian-origin rhesus macaques (Macaca mulatta) for PHS sponsored research projects. The colony is derived exclusively from Indian-origin rhesus macaques; there has been no admixture with Chinese macaques and mainland long-tailed macaques as has been reported for other breeding colonies. It has a large number of founders, and inbreeding avoidance in the population has been reported.

Post mortem services

Provides direct consultation and collaboration with veterinary clinicians and research investigators. Postmortem evaluation, which includes detailed gross and histopathological examination, is performed by a board certified anatomic pathologist. It includes collection of tissue samples for bacterial and/or viral cultures, molecular diagnostics and toxicology workup if necessary. Formalin fixed and fresh frozen tissues of all vital organs are archived. Other specialized procedures such as perfusions, interpretation of specials stains, immunohistochemistry are also available.

Training

Students are welcome to apply for internships at both Cayo Santiago and SSFS. Also as part of the One Health Initiative, the CPRC also supports outreach activities. Students from local schools participate in educational activities at the Center and are educated to understand the One Health concept.

Contact Information

Caribbean Primate Research Center Program
University of Puerto Rico Medical Sciences Campus
P.O. Box 1053
Sabana Seca, PR 00952-1053

Principal Investigator

Melween I. Martinez, DVM
Phone: 787-474-0593
Fax: 787-756-6540

Co-Investigator

Carlos A. Sariol, MD
Phone: 787-758-2525, ext. 5112 or ext. 1189
Fax: 787-767-1442
NIH Nonhuman Primate Reagent Resource
R24 OD010976

Research Emphasis/Objectives

The objective of the NIH Nonhuman Primate Reagent Resource is to facilitate the use of nonhuman primate models of disease by providing primate-specific antibody reagents for targeting cell subsets, cell receptors, soluble mediators and immunoglobulins of the most common primate species used in research.  This is accomplished by developing new reagents specific for nonhuman primate proteins or immunoglobulins and by optimizing existing reagents intended for in vitro diagnostics or for in vivo use in the nonhuman primate species.

Current Research

Current research explores methods for generating and engineering recombinant antibodies against nonhuman primate targets and expressing these antibodies at large scale.  The NIH Nonhuman Primate Reagent Resource also studies the comparative structure and function of immunoglobulins between various primate species.

Services Provided

The resource provides antibodies for in vivo administration and for in vitro diagnostics in nonhuman primates. A database of commercial reagents that cross react with 12 different nonhuman primate species is maintained on the website. The resource also provides nonhuman primate recombinant proteins, immunoglobulin reference reagents and cell lines.

Contact Information

MassBiologics
University of Massachusetts Medical School
460 Walk Hill Street
Boston, MA 02126

Principal Investigator

Keith A. Reimann, DVM
Phone: 617-474-3260
Fax: 617-474-4365

Additional Contact

Adam Buzby, MBA, Program Administrator
Phone: 617-474-3261
Fax: 617-474-4365
Squirrel Monkey Breeding and Research Resource
P40 OD010938

Research Emphasis/Objectives

Research objectives are to carry out multidisciplinary studies of reproduction in squirrel monkeys, to search for models relevant to human health, and to provide a resource of laboratory-born and -reared animals for NIH-sponsored research programs.

Current Research

Characterizing factors that influence captive reproduction with emphasis on developing methods to improve reproductive potential. A multidisciplinary approach—including behavioral studies, reproductive endocrinology, medical primatology, and genetics—is ongoing.

Services Provided

To Outside Investigators

Tissues and body fluids are available. Such specimens are provided on a priority basis to NIH-sponsored research studies that are related to the objectives of this project. Costs of packaging and shipping are negotiated on an individual basis to be determined by the nature of the collaboration.

To Collaborating Scientists

Individuals interested in collaborative studies must provide a protocol to the principal investigator. Approval of collaborative projects depends on the relevance of the proposed project to the objectives of the ongoing research effort. Complete animal husbandry, medical care, and pathology services are available without charge to investigators who have received approval from the principal investigator to use resource colony animals.

Animals

The breeding colony currently contains approximately 450 squirrel monkeys of varying ages. Some offspring and reproductive culls are available.

Contact Information

UT M.D. Anderson Cancer Center
Center for Neotropical Primate Research and Resources
Michale E. Keeling Center for Comparative Medicine and Research
Department of Veterinary Sciences
650 Cool Water Dr.
Bastrop, TX 78602

Principal Investigator and Contact

Christian R. Abee, DVM
Phone: 512-321-3991
Fax: 512-332-7312
Transgenic Huntington's Disease Monkey Resource
R24 OD010930

Research Emphasis/Objectives

Transgenic Huntington's Disease Monkey Resource (THDMR) is engaged to facilitate basic and preclinical applications of the transgenic Huntington's disease (HD) monkey model to advance scientific knowledge and the discovery of a cure for HD. The THDMR will provide a HD monkeys for investigators for collaborative study. A biomaterial bank that composes of various body fluids, blood cells, cell lines, brain sections and snap frozen tissues etc. collected throughout the lifespan of the HD monkeys are available for the biomedical research community. Longitudinal data including magnetic resonance imaging (MRI) and behavioral studies are also available for the HD research community upon request.

Current Research

Longitudinal clinical assessment of HD monkeys and cryopreservation of germ cells.

Production of second generation HD monkeys.

Animals

Research Resources: To facilitate NIH research applications of HD monkey models in basic and preclinical studies, THDMR personnel are available to help investigators design research proposals and apply for funding.

Upon receipt of funding and IACUC approval, THDMR personnel are also available to help conduct studies at the Yerkes National Primate Research Center. For approved applications, THDMR can provide up to 3 second-generation HD monkeys during a two-year time frame.

Request for Assistance: To request research proposal design assistance, investigators should submit a letter of intent with a 500-word summary that includes project title, aims and goals of the proposed study. To request on-site research assistance, please send a copy of the grant that has been funded, noting the specific research to be conducted at Yerkes.

Review criteria: The THDMR steering committee will review each letter of intent to determine alignment with the THDMR mission. Scientific merit and feasibility are key review components.

Biological Materials

The THDMR maintains a biomaterial repository with a variety of longitudinal biomaterials Yerkes personnel have collected from a cohort of transgenic Huntington's disease monkeys (x4) and age-matched control monkeys (x4). These first generation HD monkeys progressively developed HD. The available biomaterials represent infancy to adulthood and span prodromal to symptomatic stage. Biomaterials of second generation HD monkeys are also available.

See below for a sampling of biomaterials available to the HD research community. Additional biomaterial information, including HD-monkey-derived pluripotent stem cells, such as ESCS, iPSCs, NPCs and MSCs, are also available.

Longitudinal Biomaterials
The available longitudinal biomaterials are:

Plasma and serum CSF
Skin primary cultures Lymphoblast cell lines

Longitudinal MRI Data
The available longitudinal MRI data are:

T1/T2 CBF
DTI MRS

Postmortem Biomaterials
The available postmortem biomaterials available are:

Serial brain sections Snap frozen brain samples
Peripheral tissues Peripheral tissue cultures

Biomaterials Request: To request biomaterials, investigators should submit a letter of intent that includes:

  • a brief summary (no more than 500 words) on the proposed use of the biomaterials and participating investigators;
  • the amount of biomaterials you are requesting (supply is limited); and
  • your funding source/s.

Contact Information

Principal Investigator

Anthony W.S. Chan DVM Ph.D
Phone: 404-712-8347
Fax: 404-727-5289
Vervet Research Colony
P40 OD010965

Research Emphasis/Objectives

The colony emphasizes development of the Caribbean-origin vervet/African green monkey as a nonhuman primate model for biomedical research applications. The objectives are to provide SPF animals, husbandry information, access to the pedigreed, phenotyped, genotyped and genetically sequenced colony for research manipulations, and access to the tissue and data repository for hypothesis testing and pilot data generation.

Current Research

Current research includes development of data and tissue repositories from repeated assessments while consuming Western vs. usual laboratory chow diets, thereby providing a relevant nutritional context for studying chronic disease risk and facilitating ‘omic’ approaches to the study of females across the life span and juveniles of both sexes.

Services Provided

This resource will provide at least 85 animals per year for biomedical research, including some selected for particular traits (e.g., old age, insulin resistance, and impulsivity) or genotypes. Individuals wishing to collaborate on site can be provided with access to the colony for phenotypic or genetic assessment or for the conduct of discrete experiments. Technical support and complete anatomic and clinical pathology services are available. There is also scientific support for statistical genetic analyses.

Contact Information

Wake Forest University Primate Center
Wake Forest University School of Medicine
Medical Center Blvd.
Winston-Salem NC 27157-1040

Principal Investigator

Matthew J. Jorgensen, Ph.D.
Phone: 336-716-6935
Fax: 336-716-1515

Resource/Additional Contact

Jay R. Kaplan, Ph.D.
Phone: 336-716-1522
Fax: 336-716-1515

(Chimpanzee Management Program

Chimpanzee Management Program

The NIH Chimpanzee Management Program (ChiMP) supports long-term, cost-effective housing and maintenance at NIH-supported facilities for chimpanzees. ORIP provides programmatic oversight of the facilities and ensures they comply with the Animal Welfare Act, and policies concerning laboratory animal care and use.

Chimpanzee Sanctuary

In September 2002, the first award of a contract was made to Chimp Haven, Inc., a private, nonprofit organization, to establish and operate a chimpanzee sanctuary. This federal sanctuary system provides lifetime care of retired research chimpanzees as mandated by the Chimpanzees Health Improvement, Maintenance, and Protection (CHIMP) Act of December 2000.

Standards were issued to implement provisions of the CHIMP Act, which authorized the Secretary of the Department of Health and Human Services to develop and publish standards of care for chimpanzees held in the sanctuary system. The standards apply to only those facilities receiving funds as a part of the federally-funded chimpanzee sanctuary system.

October 10, 2008, Adobe Acrobat Reader IconFederal Register Notice: Standards of Care for Chimpanzees Held in the Federally Supported Chimpanzee System Exit Disclaimer.

Other facilities housing NIH-owned chimpanzees are Alamogordo Primate Facility and the National Center for Chimpanzee Care.

Chimpanzee Retirement

In November 2015, NIH announced that it will no longer support any biomedical research on chimpanzees, and that all NIH-owned and NIH-supported chimpanzees that reside outside of the Federal Sanctuary are eligible for retirement and relocation to the sanctuary as required by the Chimpanzee Health Improvement, Maintenance and Protection Act (CHIMP Act). See NIH Plan to Retire All NIH-Owned and -Supported Chimpanzees.

Costs

NIH conducts an annual census of its owned and supported chimpanzees after the end of each fiscal year (Sept. 30) to provide the public with official figures on their numbers and cost of care. For current and prior-year cost assessments, see Chimpanzee Management Reports.

See Also: Survival of Captive Chimpanzees: Impact of Location and Transfers

Contact Information

For further information, contact Sheri Hild, Ph.D.

(Specific-Pathogen-Free Macaque Resources)

Establishment of An SPF Rhesus Macaque Colony
U42 OD010442

Research Emphasis/Objectives

This program, which is located at the Southwest National Primate Research Center (SNPRC) in San Antonio, Texas, produces Indian-origin rhesus monkeys (Macaca mulatta) that are specific-pathogen-free (SPF) for herpes B virus, SIV, SRV, and STLV-1. The breeding colony produces high quality genetically characterized and MHC-typed animals for use in AIDS-related research conducted by NIH-supported grantees. Occasionally, monkeys are available for other types of research or to non-NIH funded investigators.

Services Provided

Monkeys are available for sale to investigators who want to use the monkeys in research at the SNPRC and those who want to transport the monkeys for research conducted at other sites.  ABSL-3 and ABSL-4 facilities are available at SNPRC and Texas Biomed for infectious disease research, including research with Select Agents.

Contact Information

Texas Biomedical Research Institute
Southwest National Primate Research Center
P. O. Box 760549
San Antonio, TX 78245-0549

Principal Investigator

Robert E. Lanford, Ph.D.
Phone: 210-258-9445
Maintenance of a Closed CPRC SPF Colony
U42 OD021458

Research Emphasis/Objectives

Research objective are: i) to establish, and maintain, a specific-pathogen-free (SPF) rhesus macaque supply and breeding colony program and ii) to enhance the existing specific-pathogen-free (SPF) rhesus macaque supply and breeding colony program at the SabanaSeca Field Station of the Caribbean Primate Research Center (CPRC). The CPRC program will make a significant contribution to advancing AIDS research by providing high-quality and healthy SPF rhesus monkeys to NIH-sponsored research programs. The CPRC program uses genetically characterized, MHC-typed, Indian-origin monkeys from the CPRC's free-ranging colony on the island of Cayo Santiago. Previous surveys have shown that the Cayo Santiago macaques are free of several important viruses, including retroviruses and simian virus 40 (SV-40), and that the majority of immature animals are negative for B-virus (Herpesvirussimiae or Cercopithecineherpesvirus type 1). About 20-25 percent of these monkeys are also Mamu-A*01 positive. Currently, there is a shortage of SPF rhesus monkeys for biomedical research, and the demand for these animals is expected to increase dramatically in the future. The establishment and maintenance of this program at the SabanaSeca Field Station will help meet the increased demand for both SPF (B-virus, SRV-D, SIV, STLV-1, and SV-40-free) and Mamu-A*01 positive rhesus monkeys. Forty-five SPF females and nine SPF males (nine breeding groups) will be added to the colony each year through internal recruitment from Cayo Santiago and the SabanaSeca Field Station. MHC-typing and selective breeding will be used to increase the production of SPF Mamu-A*01 positive offspring.

Current Research

The virology laboratory conducts research on recombinant DNA vaccines using rhesus macaques and performs viral tests for herpes B-virus, STLV, and SIV. The BL 2/3 Virology Laboratory has allowed CPRC to establish the SPF program under the sponsorship of ORIP, and one of its major objectives is to support ongoing SPF programs. The laboratory serves as a platform of research in vaccine development (SHIV, SIV, Dengue) and in genetics. This laboratory provides services to the conventional and SPF CPRC colonies (viral serology/PCR) .With the support of R01 and U01 NIH grants, this laboratory is collaborating with mainland investigators in vaccine and pathogenesis studies involving rhesus macaques.

Services Provided

CPRC welcomes collaborative research with established behavioral and biomedical investigators and encourages the use of its animal and osteological resources for dissertation research. Investigators are charged modest use fees for access to the animals, computerized database, and office space. All proposals receive rigorous peer review and are judged on scientific merit, feasibility, and potential overlap with ongoing studies. Protocols using live monkeys must be approved by the Institutional Animal Care and Use Committee (IACUC) of the home institution, as well as the University of Puerto Rico Medical Sciences Campus IACUC.

Contact Information

University of Puerto Rico
Medical Sciences Campus
P.O. Box 1053
Sabana Seca, PR 00952-1053

Principal Investigator

Carlos A. Sariol, M.D.
Phone: 787-758-2525 ext. 5112, or ext. 1189
Fax: 787-767-1442

Resource/Additional Contact

Virology Laboratory
Office B-315
University of Puerto Rico School of Medicine
P.O. Box 365067
San Juan, PR 00936-5067
Maintenance of an SPF Macaque Breeding Colony for AIDS Research
U42 OD010568

Research Emphasis/Objectives

The center's objective is development of a specific-pathogen-free (SPF) rhesus monkey (Macaca mulatta) breeding colony. Animals derived from the colony are limited to use for AIDS research. Nonhuman primates derived from the colony are seronegative for simian immunodeficiency virus (SIV), simian T-lymphotrophic virus (STLV-1), type D retrovirus (SRV), and Cercopithecine herpesvirus 1 (CHV1 or B-virus). The retroviruses (SIV, STLV-1, and SRV), when present in animals used for AIDS studies, may confound research data. B-virus is hazardous to humans and is being eliminated to protect personnel from coming in contact with infected animals or their tissues.

Services Provided

SPF rhesus monkeys may be requested for AIDS research by contacting the principal investigator. The Tulane Resource Allocation Committee has responsibility for allocating the animals to NIH-funded investigators. To apply for allocation of SPF rhesus monkeys must first complete an application form, which is available through the principal investigator.

Contact Information

Tulane National Primate Research Center
18703 Three Rivers Road
Covington, LA 70433

Principal Investigator

James L. Blanchard, DVM, Ph.D.
Phone: 985-871-6285

Resource/Additional Contact

Rudolf P. Bohm, Jr., DVM
Phone: 985-871-6266
Fax: 985-871-6388
Production of Pedigreed SPF Rhesus Macaques
U42 OD010990

Research Emphasis/Objectives

The objective of this program is to produce pedigreed rhesus macaques of Indian origin that are free of selected viral pathogens. These agents include: Cercopithecine herpesvirus 1 (herpes B), simian immunodeficiency virus (SIV), simian T-lymphotropic virus 1, type D retrovirus, and simian foamy virus (SFV). These animals are of known pedigrees confirmed by microsatellite testing and are also typed for Mamu-A*01 alleles by the UC Davis Veterinary Genetics Laboratory. Colony status is confirmed by frequent viral screening through the Simian Retroviral Core Laboratory.

Current Research

Research programs are in place to use assisted reproductive technology strategies to expand numbers of Mamu-A*01 positive animals and other genotypes that may be of specific research interest. The center is also identifying additional viral pathogens to be excluded from the specific-pathogen-free (SPF) population. Genetic studies are under way to determine whether A*01 positive individuals are heterozygous or homozygous.

Services Provided

Animals

Scientists wishing to use pedigreed Indian origin rhesus macaques should contact the principal investigator. Scientists wishing to conduct research at the California National Primate Research Center should contact the director. Information for research access to the primate center is available on the center's website.

Other Services

Pedigree analysis and MHC typing for rhesus macaque is available through the UC Davis Veterinary Genetics Laboratory.

Contact Information

California National Primate Research Center
University of California, Davis
One Shields Avenue
Davis, CA 95616-8542

Principal Investigator

Jeffrey A. Roberts, DVM
Phone: 530-752-6490
SPF Breeding Colonies at the Yerkes NPRC
U42 OD011023

Research Emphasis/Objectives

The Yerkes Center objective is to establish specific-pathogen-free (SPF) rhesus monkey (Macacamulatta) breeding colony at its Field Station facilities. AIDS research. The animals derived from the colony are seronegative for simian immunodeficiency virus (SIV), simian T -lymphotrophic virus (STLV-1), type D retrovirus (SRV), and Cercopithecineherpesvirus 1 (CHV1 or B-virus). In addition, all animal are genetically characterized both in respect to pedigree information and selected MHC alleles. The nonhuman primates derived from this colony are available for AIDS-related studies by NIH grantees, and all of the above listed attributes are important for AIDS research.

Services Provided

SPF rhesus macaques may be requested for AIDS research by contacting the principal investigator. The Yerkes Resource Allocation Advisory Committee has responsibility for allocating animal resources to NIH-funded investigators. To apply for allocation of SPF rhesus monkeys, investigators must first complete an application form, which is available from the principal investigator.

Contact Information

Yerkes National Primate Research Center
Emory University
954 North Gatewood Road, NE
Atlanta, GA 30322

Principal Investigator

Joyce K. Cohen, Ph.D.
Phone: 404-712-8103
Fax: 404-727-3756

Additional Contact

Maria Crane, DVM
Phone: 404-727-8653
SPF Macaca nemestrina Breeding Resource Colony
P40 OD013117

Research Emphasis/Objectives

The Specific Pathogen Free pigtailed macaque (Macaca nemestrina) breeding colony at the Johns Hopkins University serve as a national resource for this species. Pigtailed macaques are used in in several research fields including HIV/AIDS, neuroscience, immunology, teratology, behavioral sciences, psychophysics, ophthalmology, drug abuse, xenotransplantation, and reproductive system function among others.

The aims of this resource are to develop and expand a colony of pigtailed macaques (Macaca nemestrina) that are specific pathogen free (Herpes B, SRV, STLV and SIV); and which have complete pedigrees and MHC profiles. 

Current Research

Characterizing the pathogenesis of naturally occurring enterocolitis, common in this macaque species. Primary research use for these animals is the study of the pathogenesis of simian immunodeficiency virus, primarily in the central and peripheral nervous systems. Johns Hopkins’ scientists have developed an alternate method to track SIV-induced nerve fiber loss by measuring small nerve density in the sub-basal plexus of the cornea. Initial studies showed that corneal nerve fiber loss corresponded closely with loss of epidermal nerve fibers in SIV-infected animals.

Animals

The colony currently consists of 200 animals divided into single male breeding harems. Annual production is approximately 40 infants per year. The ultimate goal is to produce 100 offspring per year.

Services provided

Up to 50% of male offspring may be available for a fee to NIH-funded investigators outside of Johns Hopkins University. Blood samples and selected formalin fixed tissues may be also provided.

Contact Information/Principal Investigator

Robert J. Adams, DVM
Research Animal Resources
Ross Research 459
Johns Hopkins University
Baltimore, MD
SPF Rhesus Macaque Breeding Colony for AIDS Research
U42 OD023038
U42 OD010426

Research Emphasis/Objectives

The ONPRC maintains a specific-pathogen-free (SPF) Indian-origin rhesus macaque breeding colony in support of AIDS-related biomedical research. The research objectives are to: maintain a breeding population sufficient to supply nonhuman primates for AIDS-related research; optimize the usefulness of this population for research by characterizing ancestry, parentage, and MHC type; and ensure the population remains SPF through state of the art surveillance technologies.

Current Research

The Center's SPF Indian-origin rhesus macaque breeding programs have target female breeder populations. All adults and juveniles are free of simian immunodeficiency virus, T-lymphotrophic virus 1, type D simian retroviruses, and Herpesvirussimiae. Frequent microbiologic monitoring is performed to ensure SPF status. Research objectives focus on maintaining pedigreed, genetically diverse female breeders and production of offspring with defined MHC class I haplotypes. Polymorphic microsatellite analyses are used to verify parentage, to select appropriate males, and to monitor genetic diversity in the colonies. The SPF definition is expanded in the U42 OD023038-supported colony to include additional viral agents that are useful as models of opportunistic infections in AIDS research or as vectors for vaccine development such as cytomegalovirus, rhesus rhadinovirus, spumaretrovirus, and simian virus 40.

Services Provided

Animals

SPF juvenile Indian-origin rhesus macaques, are available for sale to NIH grantees and other qualifying investigators for AIDS-related research. Blood samples and other tissues and body fluids obtainable using routine, noninvasive clinical procedures are available to qualifying investigators with appropriate institutional approvals for research animal use. Cost estimates for collection, packaging, and shipping are available upon request. Allocation of animals produced under this cooperative agreement is determined by the ONPRC Animal Use Committee in consultation with the ORIP program administrator to ensure equitable distribution.

Other Services

The Center's resources are available to collaborative NIH grantees with appropriate animal care and use (IACUC) approval and institutional contractual agreements. Resources include veterinary clinical services, biological safety level 3 laboratory and animal containment facilities, anatomic pathology, clinical pathology, microbiology, and flow cytometry. Further information is available from the Associate Director's office. Contact Charles Roberts, Ph.D., 503-690-5259, email: robertsc@ohsu.edu.

Contact Information

Oregon National Primate Research Center
Oregon Health Sciences University, West Campus
505 N.W. 185th Avenue
Beaverton, OR 97006-3499

Grant No.: U42 OD023038
Principal Investigator

Michael K. Axthelm, DVM
Phone: 503-690-5236
Fax: 503-690-5524

Grant No.: U42 OD010426
Principal Investigator

Gregory Timmel, DVM
Fax: 503-614-3736
Tulane National Primate Research Center, AIDS SPF Breeding Colony Maintenance
U42 OD024282

Research Emphasis/Objectives

The objective of this grant is development and maintenance of a specific-pathogen-free (SPF) rhesus monkey (Macaca mulatta) breeding colony. Animals derived from the colony are limited to use for AIDS research. Nonhuman primates derived from the colony are seronegative for simian immunodeficiency virus (SIV), simian T-lymphotrophic virus (STLV-1), type D retrovirus (SRV), and Cercopithecine herpes virus 1 (CHV1 or B-virus). The retroviruses (SIV, STLV-1 and SRV), when present in animals used for AIDS studies, may confound research data. B-virus is hazardous to humans and is being eliminated to protect personnel coming in contact with infected animals or their tissues.

Services Provided

SPF rhesus monkeys may be requested for AIDS research by contacting the principal investigator. The Tulane Resource Allocation Committee has responsibility for allocating animal resources to NIH-funded investigators. To apply for allocation of SPF rhesus monkeys, investigators must first complete an application form, which is available from the principal investigator.

Contact Information

Tulane National Primate Research Center
18703 Three Rivers Road
Covington, LA 70433

Principal Investigator

Rudolf P. Bohm, Jr., DVM
Phone: 985-871-6362
Fax: 985-871-6388

Resource/Additional Contact

James L. Blanchard, DVM, Ph.D.
Phone: 985-871-6285
Washington M. nemestrina SPF Breeding Colony
U42 OD011123

Research Emphasis/Objectives

Nonhuman primate (NHP) species have proven to be invaluable resources for AIDS research in several fields, including vaccine development, therapeutic agent discovery, and pathogenesis. Well-defined models of AIDS currently exist in pigtailed macaques (M. nemestrina), utilizing a large number of primate lentiviruses and other infectious agents. SPF M. nemestrina are an essential resource for the research community as it works to address these critical issues.

A critical need exists to increase the number of pigtailed macaques available to the national research community. The Center, works with partners both within and outside NIH, has embarked on a program to increase the size of its M. nemestrina breeding colony over time, thereby increasing the supply of this resource to address important issues in research and medicine.

Current Research

Expand an M. nemestrina breeding colony free of Mycobacterium tuberculosis (TB); simian immunodeficiency virus; simian retrovirus, all subtypes; simian T-lymphoptrophic virus, and Cercopithecine herpes virus 1 for application to AIDS-related NIH research projects.

Contact Information

Washington National Primate Research Center
University of Washington Box 357330 Health Sciences Center, Room I-421 Seattle, WA 98195-7330

Principal Investigator

Michael Mustari, Ph.D.
Phone: 206-543-1430
Fax: 206-616-1710

(Nonhuman Primate Research Reagents)

NIH Nonhuman Primate Reagent Resource

R24 OD010976

Research Emphasis/Objectives

The objective of the NIH Nonhuman Primate Reagent Resource is to facilitate the use of nonhuman primate models of disease by providing primate-specific antibody reagents for targeting cell subsets, cell receptors, soluble mediators and immunoglobulins of the most common primate species used in research.  This is accomplished by developing new reagents specific for nonhuman primate proteins or immunoglobulins and by optimizing existing reagents intended for in vitro diagnostics or for in vivo use in the nonhuman primate species.

Current Research

Current research explores methods for generating and engineering recombinant antibodies against nonhuman primate targets and expressing these antibodies at large scale.  The NIH Nonhuman Primate Reagent Resource also studies the comparative structure and function of immunoglobulins between various primate species.

Services Provided

The resource provides antibodies for in vivo administration and for in vitro diagnostics in nonhuman primates. A database of commercial reagents that cross react with 12 different nonhuman primate species is maintained on the website. The resource also provides nonhuman primate recombinant proteins, immunoglobulin reference reagents and cell lines.

Contact Information

MassBiologics
University of Massachusetts Medical School
460 Walk Hill Street
Boston, MA 02126

Principal Investigator

Keith A. Reimann, DVM
Phone: 617-474-3260
Fax: 617-474-4365

Additional Contact

Adam Buzby, MBA, Program Administrator
Phone: 617-474-3261
Fax: 617-474-4365
Resource for Nonhuman Primate Immune Reagents
R24 OD010947

Research Emphasis/Objectives

The core objective of this resource is to provide information and reagents related to immunity, with a central focus on cytokines and immune cell-associated molecules, for the support of studies in NHP designed to advance understanding of immunity, immunopathology, and development of immunotherapeutic interventions. To achieve this goal the resource strives to characterize, clone, and quality testing nonhuman primate cytokines and immune cell-associated molecules in vitro and in vivo. From this work, the resource makes available to researchers working with NHP a variety of NHP cytokines, chemokines, and biological blocking reagents (e.g., sPD-1, DR5, etc.) as native or IgFc fusion proteins for their research. Finally, the resource tests NHP samples for select biological activity as a service.

Services Provided

This resource provides cDNA as well as validated DNA expression clones and recombinant proteins for many previously cloned and characterized NHP cytokines, receptors and immune mediators. Additionally the resource takes requests for the characterization of new target NHP molecules and for the production of novel custom recombinant NHP proteins (cytokines, chemokines and blocking agents). Testing of pharmacokinetics in rhesus macaques and testing of NHP samples for the biological activity of cytokines and antibodies to cytokines is also provided.

Contact Information

New Iberia Research Center
University of Louisiana at Lafayette
4401 W Admiral Doyle Drive
New Iberia, LA 70560
Phone: 337-482-0225
Fax: 337-373-0057

Principal Investigator

François Villinger, DVM, Ph.D.
Phone: 337-482-0225
Fax: 337-373-0057

Resource/Additional Contact

Kenneth Rogers PhD
Phone: 337-482-0315
Fax: 337-373-0057

Rodents

Adult Mesenchymal Stem Cell Resource

P40 OD011050

Research Emphasis/Objectives

The Adult Mesenchymal Stem/Stromal Cell Resource was established in 2003 and is now located in the Institute for Regenerative Medicine of Texas A&M Health Science Center at Scott & White. The overall objective is to provide fully characterized bone marrow-derived cells referred to as mesenchymal stem cells or mesenchymal stromal cells (MSCs) that are isolated from bone marrow, expanded and tested under standardized protocols to the scientific research community. These cells can serve as a standard against which other sources of MSCs are compared or as a source of cells for specific research applications. The preparations of human MSCs distributed must meet all the minimal defining criteria set forth by the International Society for Cytotherapy (ISCT). A key feature of the MSCs is that they are expanded at low density so that they maximally retain features of progenitor cells.

The current research involves: 1) preparing a continuous supply of human MSCs that are isolated from bone marrow aspirates provided by a variety of donors pre-screened for infectious diseases under an IRB-approved protocol with informed consent and that are thoroughly quality tested for distribution; 2) preparing a similar continuous supply of mouse bone marrow-derived MSCs for distribution; 3) preparing a similar continuous supply of rat bone marrow-derived MSCs for distribution; 4) developing improved methods of isolating and characterizing MSCs, focusing primarily on the human cells.

Services Provided

  • Currently available Adult Mesenchymal Stem/Stromal Cells for distribution include:

    1. Human MSCs from a variety of donors cultured in antibiotic-free media frozen at Passages 1 and 2 in vials of ~0.75 – 1.0 x106 cells/cryovial.
    2. Mouse MSCs isolated from male C57BL/6J and from C57BL/6-Tg(UBC-GFP)30Scha/J) mice frozen at Passage 5 in vials of ~0.5x106 cells/cryovial.
    3. Rat MSCs isolated from male Lewis rats frozen at Passage 4 or 5 in vials of ~0.5x106 cells/cryovial
  • A detailed protocol, including suggested reagents, for culturing the MSCs is provided in advance of the cell shipment.
  • A Product Specification Sheet for each lot of MSCs is included with each shipment of cells that summarizes the results of assays to characterize them, including proliferation rates, colony forming units, bi-or tri-lineage differentiation and cell surface epitope expression.
  • Technical support if needed is provided via email and/or phone for cell recipients and their staff.

Contact Information

Institute for Regenerative Medicine
Texas A&M Health Science Center College of Medicine at Scott & White
5701 Airport Road
Module C Temple, TX 76502

Principal Investigator

Darwin J. Prockop, M.D., Ph.D.
254-771-6800
Fax: 254-771-6839

Resource/Additional Contacts

Donna Tullous
254-771-6800
Fax: 254-771-6839
Roxanne L. Reger
254-771-6857
Fax: 254-771-6839
Center for Humanized Mice
R24 OD018546

Research Emphasis/Objectives

The resource offer environmentally, genetically, and xenotransplantation- engineered mouse models for translational studies and drug discovery, which are the mainstream at UNMC. The Center is focusing on improved animal models to study human immunity, human-specific infections, vaccines, and human-specific drug interactions.

The specific goals of the research are:

  • To modify mouse backgrounds for the studies of human-like adaptive immune responses to broader range of pathogens and evaluation of vaccine candidates,
  • To create strains of mice that are compatible with the function of human immune system based on human-like glycosylation patterns,
  • To modify mouse backgrounds for studies of human-like drug metabolism and drug interaction,
  • To study HIV-1-related co-infections, such as hepatitis, tuberculosis, and malaria, and
  • To examine HIV-1-associated comorbidities, including end-organ diseases like HIV-1-associated neurocognitive disorders (HAND).

Current Research

  • Develop and evaluate CD11b-DTR-NSG, CD11c-DTR-NSG, and CD11c-DTR-NSG, and CD11b/CD18m1Btlr -NSG mice optimized for human granulocyte and monocyte, dendritic cell, and endothelial cell tissue-specific distribution and function. The objective of this aim is to define the effect of knocking out mouse tissue-resident macrophages, dendritic cells, and endothelial cells, to stimulate human macrophages/endothelial cells development. Improvements over existing models will be evaluated by measuring immune responses to human vaccines in mice.
  • Distinguish the role of CMP-N-acetylneuraminic acid hydroxylase (CMAH) expression of mice in human HSC biology, and innate and adaptive immune responses. The objective of this aim is to assess the human immune cell development in Cmah knockout (KO)/NSG mice. This strain has the potential to substitute all previously developed mouse models to study human adaptive immunity.
  • Assess the impact of dual reconstitution of TK-NOG mice with human hematolymphoid and liver tissue on human innate and adaptive immune responses. The objective is to elucidate enzymatic humanization of the mouse liver in the context of hematolymphoid repopulation as an alternative approach for testing toxicology and efficacy of antiretroviral and antiviral (HIV/HCV/HBV) drugs.

Contact Information

Durham Research Center, Room 8015
985880 Nebraska Medical Center
Omaha, NE 68198-5880

Principal Investigator

Larisa Poluektova, Ph.D.
Phone: 402-559-8926 (office)

Resource/ Additional Contact

Ane Heibel
Administrative Specialist
Phone: 402-559-8754 (office)
Cre Driver Strain Resources

R24 OD011190

Research Emphasis/Objectives

The overall goal of this program is to develop and distribute comprehensive Cre strain resources and information to the scientific community. The new resources include creation, distribution and extended characterization of Cre driver lines. The program also supports the CrePortal, which leverages the informatics infrastructure of Mouse Genome Informatics to provide a database of Cre driver strains and their functionality. Together, these will provide the community with a comprehensive source of Cre driver strain functional information and animal models.

Services Provided

Animals

This program supports generation and enhancement of Cre driver strain models. This includes generation of new models from ES cell resources, generation of congenic Cre driver strains on new genetic backgrounds and deep functional characterization of these models to enhance their utility.

Information Resources

This program generates and provides primary data describing Cre driver strain functionality for strains distributed through the JAX Repository and MMRRC at JAX. This includes detailed in situ mapping of Cre strain excision activity in multiple tissues and at multiple time points. All data is provided through CrePortal (www.creportal.org), delivering a robust and complete resource for the research community.

Contact Information

The Jackson Laboratory
600 Main Street Bar Harbor, ME 04609-1500
Stephen A. Murray, Ph.D.
Phone: 207-288-6857
Fax: 207-288-6149
Janan Eppig, Ph.D.
Phone: 207-288-6422
Knockout Mouse Phenotyping Program (KOMP2)

UM 1OD023222

The Knockout Mouse Phenotyping Project (KOMP2) is a trans-NIH/Common Fund resource that performs high throughput phenotyping of knockout mouse strains. The NIH-funded centers operate in concert with other members of the International Mouse Phenotyping Consortium (IMPC) in a coordinated effort to characterize the null phenotype of every protein-coding gene. The strain data, associated metadata, raw phenotype data, output of statistical analysis, and associated annotation information are available to the worldwide community through the KOMP-funded Data Coordination Center and Database. Given that the function of a least half of the protein-coding genes is poorly understood, and that nearly one third have no functional annotation whatsoever, providing functional annotation of the mammalian genome will have a transformative effect on biology and the understanding of human disease.

The goal of the KOMP2 program is to systematically phenotype 8,500 knockout mice strains, in order to define the in vivo function of mammalian genes and to identify new models of human disease, as well as provide access to unannotated genes by providing hypothesis testing and tools. The first Phase of the Program will have succeeded in phenotyping 2500 mutant mouse lines. The current proposal is to continue the KOMP2 program, building upon the success of phase 1, and to add 3,000 more knockouts. All strains will be phenotyped as early adults (4-16 weeks), and 15% will also be ‘late adult’ phenotyped (52-72 weeks). The standardized phenotyping pipeline uses a sequence of clinical and terminal tests to identify phenotypes associated with single gene mutations. The goal is also to make the raw and annotated data available to the research community through a single, user-friendly web portal. Functional annotation of the mammalian genome will be accomplished in collaboration with the IMPC.

The most important contribution of the KOMP2 is continuous addition of new knowledge in understanding mammalian gene function using the laboratory mouse as a model. The fundamental genetic similarity between mice and humans allows researchers to infer a human gene's function based on KOMP studies. This gives insights how a similar gene in humans may contribute to disease when its activity is altered. Gene-to-phenotype associations are made by a versioned statistical analysis with all data freely available by this web portal and by several data download features.

The KOMP program has been distributing biological materials at the consistent rate of 550 unique gene knockouts per year for the last five years. Availability, accessibility, and dissemination of mice and materials into the hands of the research community are some of the principle objectives of IMPC member programs. Cooperation, coordination, and partnership among production centers and repositories over the last 5 years have resulted in knockout mouse lines for approximately 3400 genes distributed to more than 3700 PI’s at over 900 institutions in more than 30 countries on 5 continents. A significant change is that the KOMP centers will be depositing mice at the MMRRC repositories. This network brings significant additional expertise in cryopreservation, distribution, and marketing to the KOMP project, as well as a very large user network. This merger will place the KOMP resources squarely in a heavily trafficked network.

All resources generated by the KOMP resource adhere to community developed metadata standards including the use of proper allele and strain nomenclature, annotations to anatomy and phenotype ontologies, and the capture and dissemination of all metadata necessary for data reproducibility. The data is available through a standard web browser interface and through APIs.

KOMP2 is supported by the Common Fund as well contributions from the individual NIH ICs. Program is managed by the NIH Working group and administered through NHGRI and ORIP/DPCPSI. Current phase of the program is funded by three grants for production/phenotyping centers at Baylor College of Medicine (UM1HG009220), Jackson Laboratory (UM1OD023222) and University of California at Davis UM1OD023221), and one grant to the Data Coordination Center and Database (DCCDB) at European Molecular Biology Laboratory, UK (UM1HG006370).

Detailed description of the NIH funded KOMP2 initiative is described here.
IMPC data portal link

National Gnotobiotic Rodent Resource Center
P40 OD010995

Research Emphasis/Objectives

This unit provides a resource for broadly based NIH- funded investigators to examine physiologic and pathophysiologic differences in germ-free (sterile) vs. gnotobiotic (known life, selectively colonized) vs. specific pathogen-free SPF mice of different genetic backgrounds, to explore the functional alterations of normal vs. dysbiotic bacterial communities in murine models and human diseases, and to define the functional relevance of bacterial genes. The microbiota can be precisely manipulated by colonizing germ-free rodents with single or multiple resident or pathogenic bacterial, viral or fungal species using isogenic wild type or genetically-engineered bacterial strains. In addition, fecal transplants can be performed from murine models or human donors.

Services Provided:

Animals

The Center provides germ-free and selectively colonized gnotobiotic mice in multiple strains. The resource currently maintain breeding colonies of three germ-free wild type (normal) inbred mouse strains commonly used for laboratory investigations (C57Bl6/J, BALB/c, and 129S6/SvEv), Swiss Webster mice used for derivation of new mouse strains (source of surrogate mothers and vasectomized males), and 33 genetically engineered strains including those with enhanced green fluorescent protein (egfp) reporters of key pathways such as NFκBegfp and IL-10egfp TG mice.

Germ-free mouse breeding stocks:

129 S6 SvEv wild type
C57Bl/6J
BALB/c
Swiss Webster
NOD SCID
IRGM-/- (C57 BL/6)
ATG 16L1 hypomorph


IL-10 deficient (129 S6 SvEv)
Rag 2egfp
Rag 2-/- 129 S6 SvEv
RP 105-/- (C57 BL/6)
NSG
TLR 5-/- C57 BL/6
NF IL-3-/- (C57 BL/6)


IL-10-/- x Rag 2-/- C57 BL/6J
IL-10-/- x NFκBEGFP transgenic
Sccn1b-TG
IL-10egfp transgenic
NOD SCID IL-2Rγ-/- (NSG)
N/rp12-/- x Nlre5(C57 BL/6)
IL-10-/- x Rag 2-/- 129 S6 SvEv


IFNγ-/- (C57 BL/6)
ApoE-/-
AGR2-/+ (mixed)
APCMIN/IL-10-/-
NOD SCID IL-2Rγ-/- Class IIK1
P48Cre/Kras


NOD 2-/- (C57 BL/6)
NFκBEGFP transgenic
IL-10-/- (C57 BL/6
ACT-/+
PlgR-/- C57 BL/6J
Sox 9 reporter


TL1A-/-
ATG16L1T300 KI (human TG)
PI3K (C57 BL/6)
DRTSV28+/-
IL-10-/- x Wsh-/- BALB/c


Biological Materials

Tissue bank for selected tissues from germ-free C57 Bl/6J mice.

Genotyping

For relevant genetically-engineered mice only.

Training

Onsite hands-on 1-week training for investigators and technical staff planning to start a gnotobiotic facility.

Contact Information

University of North Carolina at Chapel Hill
National Gnotobiotic Rodent Resource Center
Campus Box 7032
Chapel Hill, NC 27599-7032

Principal Investigator

R. Balfour Sartor, MD
Phone: 919-966-0149
Fax: 919-843-6899

Resource Contact

Maureen Bower
Phone: 919-843-6178
Rat Resource and Research Center
P40 OD011062

Research Emphasis/Objectives

The Rat Resource and Research Center (RRRC) provides the biomedical community with ready access to valuable rat strains/stocks, embryonic stem cells and other related services that enhance the use of rats in research and shift the burden for maintaining and distributing rat models from individual investigators to a centralized repository where high standards of genetic quality control and health monitoring can be maintained to maximize research reproducibility.

Current Research

Research efforts are focused on improving cryopreservation, in vitro fertilization and artificial insemination in the rat as well as characterizing the gut microbiota to better understand its impact on model phenotypes.

Services Provided

Animals

The RRRC imports high quality, well-characterized rat strains and stocks from donating investigators using an on-line application process and active recruitment of valuable models. High demand strains are rederived to a pathogen-free state and distributed as live animals. Low demand strains are cryopreserved as germplasm and are cryorecovered upon request. All strains/stocks imported and exported from the RRRC are subjected to rigorous genetic analysis and health monitoring. The RRRC is an approved vendor for many institutions.

Biological Materials

Germline competent rat embryonic stem cell lines, including several on different genetic backgrounds and several containing EGFP are available. Germplasm (embryos and/or spermatozoa) and genomic DNA are available from rat strains/stocks. Tissue or organ samples from specific strains can be collected, preserved and/or fixed to the investigator’s specifications.

Fee-For-Service

A number of services are available on a fee-for-service basis. The RRRC encourages research collaborations with investigators and can provide consultation on colony management, husbandry, reproductive biology, gamete and embryo cryopreservation, genetics, model generation and characterization, including phenotyping, histopathology and behavioral testing.

Cryopreservation and Cryostorage

Cryopreservation and cryostorage of sperm and embryos is performed routinely for the strains/stocks donated to the RRRC and these services are available to investigators for non-RRRC strains as insurance against loss of valuable models or to generate banks of frozen material for use to refresh foundation colonies to minimize genetic drift.

Rederivation and Cryo-Resuscitation

Embryo transfer or intracytoplasmic sperm injection (ICSI) can be used to resuscitate and rederive strains/stocks. Investigators receive recovered litters with confirmed genetics and a pathogen-free health status.

Colony Management and Breeding Services

The RRRC can maintain small colonies of rats for investigators who may not have the expertise or facility space to do so. Other services include moving genetic mutations to new genetic backgrounds using a speed congenic approach, timed matings and embryo collection.

Genetic Testing

Services available for both RRRC and non-RRRC rats include, but are not limited to: genotyping (standard PCR, PCR followed by restriction endonuclease digest or nucleotide sequence analysis, RT-PCR, qPCR and probe-based allelic discrimination), sex determination assays, genotyping assay development, validation/optimization of genotyping assays, Single Nucleotide Polymorphism (SNP) analysis, speed congenic assay development, fluorescent in situ hybridization, and karyotyping.

Microinjection Services

The RRRC can perform microinjection of zygotes to produce transgenic rats or genetically modified rats using CRISPR/Cas9 genome editing technologies.

Embryonic Stem Cell Lines

The RRRC can assist with the isolation and characterization, including testing for germline competency, of new embryonic stem cells from rat strains of interest.

Pathology

Available services include, but are not limited to: pathogen detection through a collaborating diagnostic laboratory (IDEXX BioResearch), gross necropsy examination, tissue collection, and histopathologic evaluation of tissues.

Microbiota Analysis

Services available for RRRC and non-RRRC rats include, but are not limited to: targeted 16S rRNA microbiome characterization and analysis, consultation on the impact of differing microbiota on model phenotype and reproducibility, manipulation of microbiomes through rederivation or fecal transplants, and collaborative studies assessing the impact of microbiome on model phenotypes.

Training

The RRRC is affiliated with the University of Missouri (MU) Comparative Medicine Program (http://cmp.missouri.edu/ ), a training program for veterinarians pursuing careers in biomedical research. Externships for veterinary students and post-DVM trainees are available through the MU CMP.

Contact Information

Rat Resource and Research Center
Room S118, 4011 Discovery Drive, Columbia, MO 65201

Principal Investigator

Elizabeth Bryda, PhD
Phone: 573-882-5504
Fax: 573-882-9857

Resource/ Additional Resource Contact

RRRC Customer Service
Phone: 888-673-3444 or 573-884-6076

Reproductive Services

Health Monitoring and Pathology

Metagenomics

Resource for Rat Genetic Models of Aerobic Capacity
P40 OD021331

Research Emphasis/Objectives

The resource applied divergent artificial selection for intrinsic low and high endurance treadmill running capacity starting with founder population of genetically heterogeneous rats (N/NIH) and provides investigators a unique rat model system and bioanylates to explore the mechanistic basis of complex diseases. Selection across 35 generations produced lines of low capacity runners (LCR) and high capacity runners (HCR) that differ by ~8-fold in running capacity. As predicted by the Aerobic Hypothesis, disease risks segregated strongly with low aerobic capacity. The LCR score high on many risks including reduced longevity, metabolic syndrome, and Alzheimer's. The HCR score high for health factors such as resistance to obesity.

The specific goals of the resource are:

  • To make these highly valuable LCR and HCR animals available to the scientific community.
  • To continue selection of the LCR and HCR for generations 36-45 and to accumulate more recombination events that will enhance genetic mapping resolution.
  • Systematically establish bioanylate and electronic data resources and make these readily available.
  • To attract users for hypothesis-driven mechanistic study of complex diseases.

Current Research

Lines Characterization

Research is focused on enhancing the value of these rats through additional characterization and curation. Samples will be used initially for detecting genomic and transcriptomic signatures of selection and subsequently for detailed genetic profiling of the HCR and LCR lines.

Embryo Cryopreservation

Embryo cryopreservations will be performed from each of the 13 LCR and 13 HCR families at generations 37, 40, 43, and 45. This research component will generate protection for the ability of the resource to provide rats and bioanylates into the future to assure continuance and advanced development of synergistically coupled translational studies.

Contact Information

University of Michigan
Medical School
Biomedical Science Research Building 2021
109 Zina Pitcher Place
Ann Arbor, Michigan 48109-2200

Principal Investigator

Steven Loyal Britton, Ph.D.
Phone: 734 615 5969
Fax: 734 615 1722

Resource/ Additional Contact

Jane Heibel: Administrative Specialist
Phone: 734 647 1956
Fax: 734 615 3292
The Mouse Mutant Resource at the Jackson Laboratory

P40 OD010972

Research Emphasis/ObjectivesPhotograph of a brown mouse with a white underbelly

Mice harboring spontaneous mutations have long been a major source for animal models of Mendelian disease, but until recently the process of identifying causative mutations has been time-consuming and labor-intensive. Now, high-throughput DNA sequencing technologies have revolutionized the process of mutation discovery, greatly reducing the time, effort and expense associated with disease-gene identification. The Mouse Mutant Resource (MMR) at The Jackson Laboratory is leveraging these technologies to study rare phenotypic deviant mice that arise spontaneously within The Jackson Laboratory's extensive vivaria.

The MMR is the world's largest source of spontaneously arising Mendelian disease models. The resource has three key objectives:

  • To characterize — genetically, genomically and phenotypically — spontaneously arising Mendelian disease models;

  • To maintain and distribute mutant strains and associated information to the scientific community, and encourage use of these unique disease models;

  • To archive these strains for the scientific community.

Current Research

While whole exome sequencing is a powerful approach for the discovery of single nucleotide mutations and small indels (insertions deletions <50 bp) it has proven insufficient for discovery of structural mutations and copy number variations (large insertions, deletions, duplications, translocations) – which are estimated to underlie nearly 50% of Mendelian phenotypes (in mice, as well as humans). Therefore, the MMR is exploring a variety of genomic approaches to enhance the discovery of structural mutations and CNVs including comparative genome hybridization, RNASeq and whole genome sequencing.

Services Provided

The MMR offers information, resources and access to newly developed Mendelian disease models through The Jackson Laboratory. Genomic data are made available through the Mouse Mutant Resource (MMR) Database. Additional services are offered through The Jackson Laboratory: breeding and rederivation services including dedicated supply, speed expansion, congenic development, strain rescue, and microinjection; cryopreservation, storage and recovery services including sperm and embryo cryopreservation; phenotyping and pathology services; and genome sciences services, including gene mapping, genome scanning and QTL analysis.

Contact Information

JAX MMR
The Jackson Laboratory
600 Main Street
Bar Harbor, ME 04609-1500

Principal Investigator:

David Bergstrom, Ph.D.
Phone: 207-288-6609
Fax: 207-288-6149

Co-Investigator:

Laura Reinholdt, Ph.D.
Phone: 207-288-6693
Fax: 207-288-6149
Laura.Reinholdt@jax.org (link sends e-mail)
The Special Mouse Strain Resource at the Jackson Laboratory

P40 OD011102

Research Emphasis/Objectives

The Special Mouse Strains Resource (SMSR) is a resource of special strains of mice that are valuable tools for genetic analysis of complex diseases. They include panels of recombinant inbred (RI) and chromosome substitution (CS) strains. The SMSR imports, cryopreserves, and distributes RI and CS strain panels that are vital to discovery of quantitative trait loci (QTL) and ultimately genes responsible for complex diseases. QTL and genes identified by QTL analysis in mouse models often correlate with homologous regions in the human genome. Thus, connecting phenotype and genotype in mice may translate into identification of genes responsible for human complex diseases such as atherosclerosis, diabetes, obesity, asthma, autoimmunity, and hypertension.

The specific goals of the resource are:

  • To make these highly valuable RI and CS strains available to the scientific community

  • To enhance their value by additional phenotypic characterization

  • To provide all data generated by this project to the scientific community in electronic form in the Mouse Phenome Database (MPD)

Current Research

Research within the SMSR is focused on enhancing the value of these strains through additional characterization and curation. Examples are behavioral, reproductive, immunological, blood chemistries including lipids and electrolytes, and hematological studies in the C57BL/6J-Chr#A/NaJ and C57BL/6J-Chr#PWD/ForeJ consomic panels. Collected data are available from the Mouse Phenome Database (MPD) website. SNP variation data for RI strains and gene expression data for the ILSXISS RI set are also available at Mouse Phenome Database. MPD is a community resource whereby data are contributed by many different users and curated by the MPD staff at the Jackson Laboratory.

The SMSR is also deriving ES cell lines from the founder strains of the Collaborative Cross, as well as the C57BL/6J-Chr#A/NaJ CS set and in some cases, we are using RNASeq to profile gene expression in these lines. These ES cells will be useful for testing candidate genes in co-isogenic strain backgrounds, cellular phenotyping, and in vitro modeling of quantitative traits. These ES cell lines are available through the The Jackson Laboratory

The SMSR offers the following services in concert with The Jackson Laboratory: breeding and rederivation services including dedicated supply, speed expansion, congenic development, strain rescue, and microinjection; cryopreservation, storage and recovery services including sperm and embryo cryopreservation; phenotyping and pathology services; and genome sciences services, including gene mapping, genome scanning and QTL analysis.

Contact Information

JAX SMSR
The Jackson Laboratory
600 Main Street
Bar Harbor, ME 04609

Principal Investigators

Cathleen Lutz, Ph.D. (Contact PI)
Phone: 207-288-6341
Fax: 207-288-6149
Laura Reinholdt, Ph.D. (Co-PI)
Phone: 207-288-6693

(Mutant Mouse Resource and Research Centers)

Overview and Consortium Portal

The MMRRC (Mutant Mouse Resource and Research Center), the Nation’s primary mouse repository, consists of four Centers (at the University of North Carolina at Chapel Hill, University of Missouri, University of California at Davis, and The Jackson Laboratory). An Informatics, Coordination, and Service Center (ICSC) provides bioinformatics, website design and maintenance, strain acquisition coordination and curation, and customer service to help support the MMRRC repository. The MMRRCs serve to import, maintain, cryopreserve, and distribute scientifically valuable, genetically engineered mouse strains and mouse ES cell lines with potential value for the genetics and biomedical research community. Below are provided links to the major MMRRC portal to access general information, submit the strains, search MMRRC catalog and place requests. In addition, below there are descriptions of the individual MMRRC centers and links to their own websites for unique services they provide.

MMRRC portal:

https://www.mmrrc.org/

Strain submission:

https://www.mmrrc.org/submission/submIntro.php

Catalog search:

https://www.mmrrc.org/catalog/StrainCatalogSearchForm.php

Customer service:

service@mmrrc.org

(800) 910-2291 (North America) or

(530) 757-5710 (International)

Fax: (530) 757-3284

Mutant Mouse Resource and Research Center - Jackson Laboratory

U42 OD010921

Research Emphasis/Objectives

Mouse strains play a critical role in understanding the mechanisms underlying mammalian biology and human genetic disorders. These valuable resources can only be fully exploited if they are archived, managed and made accessible to the scientific community by centralized repositories. The JAX MMRRC participates with three other MMRRC distribution facilities and the Informatics, Coordination and Service Center (ICSC) to achieve this goal. The objectives of the JAX MMRRC are to: 1) Identify and evaluate biomedically-significant mice for inclusion in the MMRRC program; 2) Import/acquire and archive mouse strains into a high quality, secure storage facility using state of the art rederivation methods to eliminate specific pathogens, and advanced cryopreservation techniques to safely store germplasm and embryos; 3) Distribute mouse strains in the form of live mice, cryopreserved germplasm or embryos in addition to cryopreserved ES cell lines; 4) Operate a state-of-the-art quality control program to ensure genetic stability by monitoring mutant genotype, mutant phenotype, and genetic background; and to protect animal health by monitoring material tracking, pathogen testing, and standard operating procedures (SOPs) in Repository mouse rooms.

Current Research

Research efforts at the JAX MMRRC are aimed at optimization and implementation of CRISPR/cas9 based genome editing in zygotes and ES cell lines across a broad range of inbred strain backgrounds. Opportunities for collaborative, CRISPR/cas9 based model development projects are available.

Services Provided

The MMRRC at JAX offers the following services in concert with The Jackson Laboratory: breeding and rederivation services including dedicated supply, speed expansion, congenic development, strain rescue, and microinjection; cryopreservation, storage and recovery services including sperm and embryo cryopreservation; phenotyping and pathology services; and genome sciences services, including gene mapping, genome scanning and QTL analysis.

Contact Information

JAX MMR
The Jackson Laboratory
600 Main Street
Bar Harbor, ME 04609-1500

Principal Investigator

Cathleen Lutz, Ph.D.
Phone: 207-288-6341
Fax: 207-288-6149

Resource/ Additional Contact

Laura Reinholdt, Ph.D. (Co-PI)
Phone: 207-288-6693
Fax: 207-288-6149
 
Steve Rockwood
Phone: 207-288-6437
Fax: 207-288-6149
Mutant Mouse Resource and Research Center - University of Missouri

U42 OD010918

The Mutant Mouse Resource and Research Center (MMRRC) at the University of Missouri (MU-MMRRC) was established in 2000. The MU-MMRRC is a consortium of investigators including Craig Franklin, DVM, PhD; Elizabeth Bryda, PhD; Yuksel Agca, DVM, PhD, Jim Amos-Landgraf, PhD; and Aaron Ericsson, DVM, PhD at the University of Missouri.

Research Emphasis/Objectives

Research projects focus on areas of major importance to mice as animal models including: 1) refinement of models to ensure study reproducibility through characterization of intestinal microbiota and its contribution to model phenotypes, and 2) development and improvement of economical methods for cryopreservation of mouse strains. Toward the completion of Objective 1, studies aim to characterize the influence of multiple common husbandry variables (genetic background, animal source, caging, bedding, food) to the composition of the gut microbiota, and to demonstrate that husbandry-induced alterations in the microbiota can significantly affect model outcomes. Toward the completion of Objective 2, studies aim to refine and improve methods of sperm cryosurvival via manipulation of cooling and warming rates in the presence of the compound iodixanol.

Services Provided

Animals

The overall goal is to distribute high-quality, well-characterized inbred, hybrid, and mutant mice to investigators. To this end, the MMRRC selects and imports mouse strains and stocks important to the biomedical research community, rederives mice to a pathogen-free state, cryopreserves gametes and embryos, performs genotyping and infectious disease monitoring to ensure the quality of the mice, and distributes mice to investigators as live mice.

Biological Materials

In addition to live animals, cryopreserved germplasm, genomic DNA or tissue samples from models can be provided.

Fee-For-Service

A number of services are available on a fee-for-service basis. The MMRRC encourages research collaborations with investigators and can provide consultation on colony management, husbandry, reproductive biology, gamete and embryo cryopreservation, genetics, model generation and characterization, including phenotyping, histopathology and behavioral testing.

Cryopreservation and Cryostorage

Cryopreservation and cryostorage of sperm and embryos is performed routinely for the strains/stocks donated to the MMRRC and these services are available to investigators for non-MMRRC strains as insurance against loss of valuable models or to generate banks of frozen material for use to refresh foundation colonies to minimize genetic drift.

Rederivation and Cryo-Resuscitation

Embryo transfer or intracytoplasmic sperm injection (ICSI) can be used to resuscitate and rederive strains/stocks. Investigators receive recovered litters with confirmed genetics and a pathogen-free health status.

Colony Management and Breeding Services

The MMRRC can maintain small colonies of mice for investigators who may not have the expertise or facility space to do so. Other services include moving genetic mutations to new genetic backgrounds using a speed congenic approach, timed matings and embryo collection.

Genetic Testing

Services available for both MMRRC and non-MMRRC mice include, but are not limited to: genotyping (standard PCR, PCR followed by restriction endonuclease digest or nucleotide sequence analysis, RT-PCR, qPCR and probe-based allelic discrimination), sex determination assays, genotyping assay development, validation/optimization of genotyping assays, Single Nucleotide Polymorphism (SNP) analysis, speed congenic assay development, fluorescent in situ hybridization, and karyotyping.

ES Cell and Microinjection Services

Services available for MMRRC mice as well as non-MMRRC mice include, but are not limited to: electroporation and selection in ES cells, genome editing (e.g., CRISPR/Cas9), and transgene development (e.g., plasmid, BAC).

Services available for MMRRC mice as well as non-MMRRC mice include, but are not limited to: pathogen detection through a collaborating diagnostic laboratory (IDEXX BioResearch), gross necropsy examination, tissue collection, and histopathologic evaluation of tissues.

Pathology

Services available for MMRRC mice as well as non-MMRRC mice include, but are not limited to: targeted 16S rRNA microbiome characterization and analysis, consultation on the impact of differing microbiota on model phenotype and reproducibility, manipulation of microbiomes through rederivation or fecal transplants, and collaborative studies assessing the impact of microbiome on model phenotypes.

Training

The MU MMRRC is affiliated with the Comparative Medicine Program, a training program for veterinarians pursuing careers in biomedical research. Externships for veterinary students and post-DVM trainees are available through the MU CMP.

Contact Information

University of Missouri — MMRRC
4011 Discovery Drive
Columbia, MO 65201

Principal Investigator

Craig Franklin, DVM, PhD, DACLAM
Phone: 573-882-6623
Fax: 573-882-9857

Resource/ Additional Contact

MMRRC Customer Service
Phone: 888-673-3444 or 573-884-6076
Fax: 573-882-9857
Reproductive Services
Yuksel Agca, DVM, PhD
Genetics and Genotyping
Elizabeth Bryda, PhD
Metagenomics
Aaron Ericsson, DVM, PhD
Mutant Mouse Resource and Research Center - University of California, Davis

U42 OD012210

Research Emphasis/Objectives

The Mutant Mouse Resource and Research Center (MMRRC) at UC Davis imports, archives, maintains, and distributes more than 30,000 mutant mouse alleles as live mice, frozen germplasm, stem cells, and molecular vectors for use in biomedical research. The MMRRC Davis receives transgenics, knockouts, and other kinds of mutant mouse lines at no cost to the donor, and after re-derivation and cryopreservation, distributes breeding stock, germplasm, cells, or tissues of genetically-defined and pathogen-free mice for a small fee to requesting investigators. The MMRRC also serves an integral role in the UC Davis Mouse Biology Program (MBP), a center of scientific excellence in mouse biology, genetic engineering and CRISPR/Cas genome editing, genetic and genotyping services, strain rescue, pathology and clinical pathology phenotyping, behavioral phenotyping, non-invasive imaging, and infectious disease diagnostics.

Services Provided

Gene targeting, CRISPR/Cas9 genome editing, cryopreservation, rederivation, strain rescue, construct design, random and targeted mutations, speed congenics, microinjection, aggregation, genotyping, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), pathology and behavioral phenotyping, diagnostics, experimental pathology, non-invasive imaging and image analysis, bioinformatics and data analysis, colony management, and teaching resources.

Contact Information

Mutant Mouse Resource and Research Center
c/o Mouse Biology Program
University of California, Davis
2795 Second Street, Suite 400
Davis, CA 95618

Principal Investigator

K. C. Kent Lloyd, DVM, Ph.D
Phone: 530-754-6677
Fax: 530-757-3277

Resource/ Additional Contact

Associate Director
Kristin Evans, DVM, Ph.D
Phone: 530-754-6687
Fax: 530-757-3277
MMRRC Repository Manager
Renee Araiza
Phone: 530-754-6677 or 530-757-8476
Fax: 530-757-3284
Mutant Mouse Resource and Research Center - University of North Carolina

U42 OD010924

Research Emphasis/Objectives

The Mutant Mouse Regional Resource Center at University of North Carolina (MMRRC-UNC) is a mouse cryoarchive and distribution center, which incorporates research goals that synergize with and extend the value of the resource. The goals of the UNC Chapel Hill center are to 1) streamline and improve operating procedures to increase importation, distribution, and cryoarchiving of mouse strains; 2) establish a comprehensive cryoarchive for the Collaborative Cross (CC) resource; 3) develop and disseminate computational tools for mouse genotyping and genomics research, which will enhance phenotypic reproducibility; 4) examine the effect of paternal age and epigenetics on mutation rate.

Current Research

It is widely recognized that genetic background can have a great impact on the interpretation and reproducibility of data. A main area of research at the MMRRC-UNC is focused on the development of genomic and computational tools to characterize the genetic background of the mouse models to better understand the genetic underpinnings of disease and enhance experimental standardization. A second area of research is the characterization of the paternal age effect (PAE) on mutation rate. PAE is an important biological question that has bearing on both evolutionary genetics and on the incidence and etiology of human diseases. PAE also has the potential to have a dramatic effect on genetic drift of mouse stocks. Therefore, PAE is an important consideration for standard operating procedures (SOP) of collecting and preserving sperm by investigators and repositories.

Services Provided

Maintain selected strains of genetically modified mice; distribute mutant mice and collaborative cross strains from live colony or cryopreserved archive; distribute frozen sperm and frozen embryos; health testing; cropreservation and re-derivation; intracytoplasmic sperm injection; support for the development and application of genetic validation tools utilizing mouse universal genotyping data.

Contact Information

MMRRC-Chapel Hill
5023E Genetic Medicine Building
120 Mason Farm Road, CB 7519
Chapel Hill, NC 27599

Principal Investigator

Terry Magnuson, Ph.D.
Phone: 919-843-6475
Fax: 919-843-6365

Resource Contact

Virginia Godfrey, DVM, Ph.D.
Phone: 919-966-2903
Fax: 919-962-4296

Swine

National Swine Resource and Research Center

U42 OD011140

Research Emphasis/Objectives

The National Swine Resource and Research Center (NSRRC) was established in 2003 to develop the infrastructure to ensure that biomedical investigators across a variety of disciplines have access to critically needed swine models of human health and disease. The NSRRC will also serve as a central resource for reagents, creation of new genetically modified swine, and information and training related to use of swine models in biomedical research.

Services Provided

Animals

Swine are imported into the resource center, rederived to a pathogen-free status, and gametes and embryos are cryopreserved to prevent future loss. Swine models are then available for distribution to biomedical investigators at a nominal cost.

Biological Materials

Tissues/organs

Health Monitoring

The NSRRC performs on-going health monitoring to assure maintenance of a pathogen-free status.

Cryopreservation

The NSRRC cryopreserves and stores gametes, embryos and somatic cells to prevent loss of valuable models

Research

  • Improved techniques for cryopreservation of pig reproductive cells and tissues
  • Improved methods for the detection and elimination of microbial pathogens in pigs
  • Development of improved methods for the production of transgenic and knockout pig

PI-Driven Model Creation

The center creates custom models for NIH-funded investigators. Requests are evaluated by the Steering Committee and prioritized. Animals are first distributed to the requesting principal investigators, and then made available to others.

Contact Information

WBC 107 Animal Science Research Center
University of Missouri
920 East Campus Drive
Columbia, MO 65211

Principal Investigator

Randall S. Prather, Ph.D.
University of Missouri
Animal Science Research Center
Phone: 573-882-7446

Resource Contact

Eric M. Walters, Ph.D.
University of Missouri
Animal Science Research Center
Phone: 573-882-1134