Selected Grantee Publications
- Clear All
- 2 results found
- New Approach Methodologies
- 2023
Ion Channel Function in Translational Bovine Gallbladder Cholangiocyte Organoids: Establishment and Characterization of a Novel Model System
Nagao and Ambrosini et al., Frontiers in Veterinary Science. 2023.
https://pubmed.ncbi.nlm.nih.gov/37303723/
The study of biliary physiology and pathophysiology has long been hindered by the lack of in vitro models that accurately reflect the complex functions of the biliary system. Recent advancements in 3D organoid technology may offer a promising solution to this issue. Bovine gallbladder models have recently gained attention in the investigation of human diseases due to their remarkable similarities in physiology and pathophysiology to the human gallbladder. In this study, the investigators successfully established and characterized bovine gallbladder cholangiocyte organoids (GCOs) that retain key characteristics of the gallbladder in vivo, including stem cell properties and proliferative capacity. Notably, their findings demonstrate that these organoids exhibit specific and functional cystic fibrosis transmembrane conductance regulator activity. These bovine GCOs represent a valuable tool for studying the physiology and pathophysiology of the gallbladder with human significance. Supported by ORIP (K01OD030515, R21OD031903).
CD8+ T Cells Promote HIV Latency by Remodeling CD4+ T Cell Metabolism to Enhance Their Survival, Quiescence, and Stemness
Mutascio et al., Immunity. 2023.
https://www.doi.org/10.1016/j.immuni.2023.03.010
An HIV reservoir persists following antiretroviral therapy, representing the main barrier to an HIV cure. Using a validated in vitro model, investigators explored the mechanism by which CD8+ T cells promote HIV latency and inhibit latency reversal in HIV-infected CD4+ T cells. They reported that CD8+ T cells favor the establishment of HIV latency by modulating metabolic, stemness, and survival pathways that correlate with the downregulation of HIV expression and promote HIV latency. In future studies, comparative analyses may provide insight into common molecular mechanisms in the silencing of HIV expression by CD8+ T cells and macrophages, which can be applied to new intervention strategies that target the HIV reservoir. Supported by ORIP (P51OD011132, S10OD026799), NIAID, NIDDK, NIDA, NHLBI, and NINDS.