Selected Grantee Publications
- Clear All
- 2 results found
- New Approach Methodologies
- T32
Matrikine Stimulation of Equine Synovial Fibroblasts and Chondrocytes Results in an In Vitro Osteoarthritis Phenotype
Gagliardi et al., Journal of Orthopaedic Research. 2025.
https://pubmed.ncbi.nlm.nih.gov/39486895
Advancements in therapy development for osteoarthritis (OA) currently are limited due to a lack of physiologically relevant in vitro models. This study aimed to understand the effect of matrikine stimulation, using human recombinant fibronectin fragment containing domains 7–10 (FN7–10), on equine synovial fibroblasts and chondrocytes. Inflammatory cytokines, chemokines, and matrix degradation genes in equine synovial fibroblasts and chondrocytes were significantly altered in response to FN7–10 stimulation; marked upregulation was observed in interleukin-6 (IL-6), IL-4, IL-10, matrix metalloproteinase 1 (MMP1), MMP3, MMP13, CCL2/MCP1, and CXCL6/GCP-2 gene expression. Only IL-6 protein production was significantly increased in media isolated from cells stimulated with FN7–10. These results support the potential use of equine synovial fibroblasts and chondrocytes—employing FN7–10—as representative in vitro models to study OA. Supported by ORIP (T32OD011130) and NIAMS.
Controlled Stiffness of Direct-Write, Near-Field Electrospun Gelatin Fibers Generates Differences in Tenocyte Morphology and Gene Expression
Davis, Journal of Biomechanical Engineering. 2024.
https://pubmed.ncbi.nlm.nih.gov/38529730/
Tendinopathy is associated with mobility issues, and the cell–matrix interactions involved in the development of tendinopathy are not fully understood. In this study, researchers fabricated micron-scale fibrous scaffolds that mimic native collagen fiber size and orientation. They reported that matrix metalloproteinase and proteoglycans (possible indicators of tendinopathy) were more upregulated in the presence of high-stiffness fibers than low-stiffness fibers. The authors’ findings suggest that scaffolds can serve as in vitro models for tendinopathy. Additionally, their work suggests that matrix mechanical properties contribute to cell–matrix interactions during tendinopathy formation. Supported by ORIP (T32OD011130) and NIA.